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Cat. No.: PE2-500 (for 500pmol)

Cat. No.: PE2-2500 (for 2500pmol)

Description

PE2 (Prime Editor 2) is the core component of the pioneering prime‑editing system, enabling precise genome modifications without introducing DNA double‑strand breaks. PE2 is a fusion protein composed of the SpCas9(H840A) nickase and an engineered Moloney murine leukemia virus reverse transcriptase (M‑MLV RT). The Cas9 module carries the H840A point mutation, which specifically inactivates the HNH nuclease domain while retaining RuvC activity. As a result, the enzyme introduces a single‑strand nick on the non‑complementary DNA strand under pegRNA guidance.

The fused M‑MLV reverse transcriptase contains five key amino‑acid substitutions that significantly enhance its thermostability, processivity, and template‑binding affinity. These improvements allow PE2 to efficiently synthesize the edited DNA sequence directly at the nick site.

Prime editing enables all 12 possible base‑to‑base conversions, as well as precise small insertions and deletions, without requiring donor DNA templates or generating hazardous double‑strand breaks. This greatly reduces the risk of off‑target mutations and chromosomal rearrangements. Compared with the first‑generation PE1 system, PE2 achieves a 2.3‑ to 5.1‑fold increase in editing efficiency due to the optimized reverse transcriptase, establishing a strong foundation for broad applications in basic research, gene therapy, and crop engineering.