Bst DNA/RNA Polymerase is a mixture of Bst polymerase and extremely thermostable reverse transcriptase (65°C tolerant), which is suitable for the isothermal amplification reaction of RNA. It can detect low-sensitivity RNA molecules. This enzyme is recommended in isothermal amplification experiments using RNA as a template. In addition, Bst DNA/RNA Polymerase can also perform isothermal amplification of DNA templates.
Our Bst DNA/RNA Polymerase is included in New Products, Science Journal, December 2, 2021.
At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase.
Bst DNA/RNA Polymerase is suitable for isothermal amplification reaction of both DNA and RNA templates, which can detect low-sensitivity nucleic acid templates with great efficiency and specificity. Besides, with a special preparation process, this enzyme has a fast amplification rate and high tolerance to impurity. Since our Bst DNA/RNA Polymerase is extremely thermostable and also provides sensitive reverse transcriptase activity, it is reported to have higher sensitivity at high Ct values.
 Lu S, Duplat D, Benitez-Bolivar P, León C, Villota SD, Veloz-Villavicencio E, et al. (2022) Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application. PLoS ONE 17(5): e0268340. https://doi.org/10.1371/journal.pone.0268340
DNA/RNA isothermal amplification
GC-rich rapid sequencing
Rapid sequencing of micro-template DNA
Strong Recognition Ability to dUTP
Bst DNA/RNA polymerase has a strong recognition ability to dUTP. The dTTP needed for amplification reaction can be completely replaced by dUTP, so the amplification products all contain dUTP. By adding our Heat-Labile Uracil DNA Glycosylase (HL-UDG), aerosol pollutants will be completely removed in the initial reaction stage. The HL-UDG can be later inactivated irreversibly within 3 min at 65°C, which not only eliminates the pollutants but also ensures the normal amplification of nucleic acid. Therefore, the false positive caused by aerosol pollution in the reaction can be greatly reduced.
Store the components at -20°C. Avoid multiple freeze-thaw cycles.
We provide robust and reliable solutions for the study of various diseases (African Swine Fever,COVID-19, etc) based on Bst DNA/RNA Polymerase, please contact us or email firstname.lastname@example.org for more details.
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
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At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase. Based on this special enzyme, we have developed PrimeIampTM lyophilized isothermal amplification microbeads series. These series are ready-to-use master mixes, which can perform isothermal amplification directly when the templates and primers are added. With freeze-drying technology, these master mixes are lyophilized into solid microbeads, which can be transported and stored at room temperature with great convenience.
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