Cat. No.: IAMBMB-100 (for 100T)
Cat. No.: IAMBMB-1k (for 1,000T)
PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads contain reaction buffer, Mg2+, dNTP, Bst DNA/RNA polymerase and so on in lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. As the basic version of PrimeIAmp™ Lyophilized Isothermal Amplification Microbeads series, this version does not contain any dyes and can be flexibly used for various isothermal amplification applications. PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for 6 months.
Minimum shelf life is 6 month at 25°C and 3 years at 2-8°C. The product can be transported at room temperature. After the product is opened, the unused product should be sealed with sealing film or electrical tape to prevent the performance degradation due to moisture. The product can also be vacuum packed in 0.2 ml EP tube.
- We recommend use ddH2O to preserve samples for detection when using crude samples. The best crude sample is swab sample. Swab sample soaked in ddH2O can be directly used as template for amplification without nucleic acid purification.
- When used in other isothermal amplification reactions (such as CPA, SMAP, etc.), the above strategies can be referred while the reaction time may need to be adjusted.
- After the reaction system is prepared, a drop of mineral oil can be added to cover the upper part of the reaction liquid to reduce aerosol pollution.
- Preparation of complete amplification system using lyophilized microbeads: Pack optimized primers at the bottom of 0.2 ml EP tube and dry at 70 ~ 80 °C for 1 ~ 2h. The dried 0.2 ml EP tube already contains dried amplification primers, and then add a lyophilized microbead to prepare the complete amplification system, which can be stored for a long time, without the need of cold chain transportation.
Instruction: Protocol (take PrimeIAmp™ Red Lyophilized Isothermal Amplification Microbeads as an example)
Related: PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads, PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads
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SBS Genetech: Leading the Next Generation Isothermal Amplification Technology
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory