All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: IAMBMB-100 (for 100T)

Cat. No.: IAMBMB-1k (for 1,000T)

Cat. No.: IAMBMB-10k (for 10,000T)

Cat. No.: IAMOG-96 (for OG Colorimetric Tube (96T))

Please note that PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads don't include OG Colorimetric Tube. If OG Colorimetric Tube is required, please include it when placing the order.

Description

PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads contain reaction buffer, Mg²⁺, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. Like the basic version of  PrimeIAmp™ Lyophilized Isothermal Amplification Microbeads series, this version does not contain any dyes and can be flexibly used for various isothermal amplification applications (e.g. OG Colorimetric Tube). PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for more than 6 months.

OG Colorimetric Tube (Optional)

The orange-green colorimetric reaction is carried out by OG Colorimetric Tube, and the lyophilized OG dye is pre-added to the caps of the 8-strip tube. After isothermal amplification, reverse OG Colorimetric Tube to dissolve OG dye. The positive sample will turn bright green while the negative sample will turn dark orange, which can be observed by naked eyes.

Note: PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads don't include OG Colorimetric Tube. If OG Colorimetric Tube is required, please include it when placing the order.

Storage

The minimum shelf life is 6 months at 25°C and 2 years at -20°C. The product can be transported at room temperature. After the product is opened, the unused product should be sealed with sealing film or electrical tape to prevent performance degradation due to moisture. The product can also be vacuum packed in a 0.2 ml EP tube.

Note

• When used in other isothermal amplification reactions (such as CPA, SMAP, etc.), the above strategies can be referred to while the reaction time may need to be adjusted.
• After the reaction system is prepared, a drop of mineral oil can be added to cover the upper part of the reaction liquid to reduce aerosol pollution.
• Preparation of complete amplification system using lyophilized microbeads: Pack optimized primers at the bottom of 0.2 ml EP tube and dry at 70 ~ 80 °C for 1 ~ 2h. The dried 0.2 ml EP tube already contains dried amplification primers, and then add a lyophilized microbead to prepare the complete amplification system, which can be stored for a long time, without the need for cold chain transportation.

PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads is included in New Products, Science Journal, February 3, 2022. Please visit: https://www.science.org/doi/10.1126/science.ada0688

Instruction: Protocol

Related: 

Lyophilized beads based on Bst DNA Polymerase

• LAMP Basic Lyophilized Microbeads
• RT-LAMP Basic Lyophilized Microbeads
• RDA (RNase H Depended Amplification) Lyophilized Microbeads

Lyophilized beads based on WarmUp Bst DNA Polymerase (upgraded version of Bst DNA Polymerase)

• WarmUp LAMP Lyophilized Microbeads
• WarmUp RT-LAMP Lyophilized Microbeads
• WarmUp RDA (RNase H Depended Amplification) Lyophilized Microbeads

Lyophilized beads based on Bst DNA/RNA Polymerase

• PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads
• PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads, 
• PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads

Lyophilized beads based on Bst P DNA/RNA Polymerase (upgraded version of Bst DNA/RNA Polymerase)

• PrimeIAmp™ HotStart Bst P Basic Microbeads
• PrimeIAmp™ HotStart Bst P SYBR Green Microbeads
• PrimeIAmp™ HotStart Bst P L-HNB Microbeads

Viewpoint

Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.

SBS Genetech: Leading the Next Generation Isothermal Amplification Technology

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory