Both DNA and RNA samples can be used for isothermal amplification with PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads. With freeze-drying technology, PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads can be transported and stored at room temperature.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: IAMBMB-100 (for 100T)
Cat. No.: IAMBMB-1k (for 1,000T)
Cat. No.: IAMOG-48 (for OG Colorimetric Tube (48T))
Please note that PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads don't include OG Colorimetric Tube. If OG Colorimetric Tube is required, please include it when placing the order.
PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads contain reaction buffer, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. Like the basic version of PrimeIAmp™ Lyophilized Isothermal Amplification Microbeads series, this version does not contain any dyes and can be flexibly used for various isothermal amplification applications (e.g. OG Colorimetric Tube). PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for 6 months.
OG Colorimetric Tube (Optional)
The orange-green colorimetric reaction is carried out by OG Colorimetric Tube, and the lyophilized OG dye is pre-added to the caps of the 8-strip tube. After isothermal amplification, reverse OG Colorimetric Tube to dissolve OG dye. The positive sample will turn bright green while the negative sample will turn dark orange, which can be observed by naked eyes.
The minimum shelf life is 6 months at 25°C and 2 years at 2-8°C. The product can be transported at room temperature. After the product is opened, the unused product should be sealed with sealing film or electrical tape to prevent performance degradation due to moisture. The product can also be vacuum packed in a 0.2 ml EP tube.
When used in other isothermal amplification reactions (such as CPA, SMAP, etc.), the above strategies can be referred to while the reaction time may need to be adjusted.
After the reaction system is prepared, a drop of mineral oil can be added to cover the upper part of the reaction liquid to reduce aerosol pollution.
Preparation of complete amplification system using lyophilized microbeads: Pack optimized primers at the bottom of 0.2 ml EP tube and dry at 70 ~ 80 °C for 1 ~ 2h. The dried 0.2 ml EP tube already contains dried amplification primers, and then add a lyophilized microbead to prepare the complete amplification system, which can be stored for a long time, without the need for cold chain transportation.
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
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