PrimeIAmp™ HotStart Bst P L-HNB Microbeads
$700.00 - $6,300.00
All products have special prices for bulk purchase, please contact us for more details if required.
Cat. No.: ILHPMB-100 (for 100T)
Cat. No.: ILHPMB-1k (for 1,000T)
PrimeIAmp™ HotStart Bst P L-HNB Microbeads is a full-system lyophilized microbead product, which contains Bst P DNA/RNA Polymerase, L-HNB (Leuco-HNB) colorimetric dye, Helicase, dNTP, Mg2+, reaction buffer salt, lyophilized excipient. The amplification system is 25μl for each microbead.
L-HNB dye in the product combines with Mg2+ to produce deep violet color before the reaction starts. With the LAMP reaction, the resulting PPi (inorganic pyrophosphate) combines with Mg2+ in the system, making L-HNB show light green.
- The whole Bst P DNA/RNA System includes hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic facilitates the establishment of the reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures.
- The reaction temperature is further raised to 70°C, which greatly reduces the formation of primer dimer, improves the amplification specificity, and makes the nucleic acid release of crude samples more sufficient.
- The whole portfolio contains Helicase, so Premium LAMP amplification (pLAMP) is allowed without using F3/B3 primers. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This will further reduce non-specific amplification and greatly improve amplification homogeneity.
The minimum shelf life is 24 months at -20°C when sealed. The minimum shelf life is 12 months at room temperature (25°C) when sealed. Avoid moisture.
Related: Bst P DNA/RNA Polymerase
Customized Lyophilized Microbead Service
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