Both DNA and RNA samples can be used for isothermal amplification with PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads. With freeze-drying technology, PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads can be transported and stored at room temperature.
PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads contain reaction buffer, SG fluorescent dye, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. SG fluorescent dye is specially designed for real-time fluorescence detection. PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for 6 months.
The minimum shelf life is 6 months at 25°C and 3 years at 2-8°C. The product can be transported at room temperature. After the product is opened, the unused product should be sealed with sealing film or electrical tape to prevent performance degradation due to moisture. The product can also be vacuum packed in a 0.2 ml EP tube.
When used in other isothermal amplification reactions (such as CPA, SMAP, etc.), the above strategies can be referred to while the reaction time may need to be adjusted.
After the reaction system is prepared, a drop of mineral oil can be added to cover the upper part of the reaction liquid to reduce aerosol pollution.
Preparation of complete amplification system using lyophilized microbeads: Pack optimized primers at the bottom of 0.2 ml EP tube and dry at 70-80°C for 1-2h. The dried 0.2 ml EP tube already contains dried amplification primers, and then add a lyophilized microbead to prepare the complete amplification system, which can be stored for a long time, without the need for cold chain transportation.
There is no detergent in the product. Adding Tween20 with a final concentration of 0.5% to the reaction system will accelerate the reaction speed for about 3min. Preparation method of Tween20 with 10× concentration: Tween20 is dissolved in ddH2O with a concentration of 5%, and the pH is adjusted to 8.8 with dilute NaOH solution (50mM). The solution is used as 10×, and 2.5μl is used for each microbead.
Under the condition of low-temperature storage, before opening the cap, the bottle shall be placed at room temperature to make the temperature of the lyophilized microbeads to be consistent with the room temperature, which can prevent moisture in the air from being absorbed by microbeads due to the difference in temperature.
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.
Peptides are synthesized by the condensation reaction of the carboxyl group of one amino acid with the amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development, and polypeptide vaccine development. Each of our polypeptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis with high quality.
A peptide library is a new technique for studying the structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.
Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.
Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, Pfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. High-quality dNTPs and NTPs (set, mix) are also supplied.
Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide RNasin (RNase inhibitor) and M-MLV reverse transcriptase to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series is designed for the isolation of viral RNA/DNA or bacterial DNA.
At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase. Based on this special enzyme, we have developed PrimeIampTM lyophilized isothermal amplification microbeads series. These series are ready-to-use master mixes, which can perform isothermal amplification directly when the templates and primers are added. With freeze-drying technology, these master mixes are lyophilized into solid microbeads, which can be transported and stored at room temperature with great convenience.
RNA silencing technology has become a powerful tool to study gene function. The success of any RNA experiment depends on high-quality siRNA and effective transfection reagent. With chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro. As for transfection reagent, our ready-to-use siRNA transfection reagent, Sirnafectamine, can be used for a wide range of cell lines, with minimal cytotoxicity and the best cell state after transfection. As Sirnafectamine will protect RNA during the whole process, a very low concentration of siRNA can produce high gene silencing efficiency.
Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column) and magnetic beads technology (VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA/RNA from various sources quickly and reliably. The purity of DNA/RNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning, and cleavage.
DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. The gene-editing technology based on this system has a wide variety of applications. Here, we provide various Cas nucleases, synthetic sgRNAs, and T7 Endonuclease I of high quality to improve the accuracy and efficiency of your experiments.
Gene manipulation is a process that uses biotechnology to manipulate genes directly to generate new DNA and has been widely used in research, medicine, industrial biotechnology, and agriculture. Our Premium™ Master Assembly Mix and Topo Cloning kits (pBM23, pBM16A, pBM16K) provide efficient solutions for these types of demands.
GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.
A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of an organism, often a virus or bacteria that acts as a pathogen in blood, tissue, urine, etc. Based on our leading fluorescent quantitative PCR technique and isothermal amplification technology, we have developed solutions for various pathogens.