Both DNA and RNA samples can be used for isothermal amplification with PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads. With freeze-drying technology, PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads can be transported and stored at room temperature.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: IAMSGMB-100 (for 100T)
Cat. No.: IAMSGMB-1k (for 1,000T)
PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads contain reaction buffer, SG fluorescent dye, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. SG fluorescent dye is specially designed for real-time fluorescence detection. PrimeIAmp™ SG Fluorescent Dye Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for 6 months.
The minimum shelf life is 6 months at 25°C and 2 years at -20°C. The product can be transported at room temperature. After the product is opened, the unused product should be sealed with sealing film or electrical tape to prevent performance degradation due to moisture. The product can also be vacuum packed in a 0.2 ml EP tube.
When used in other isothermal amplification reactions (such as CPA, SMAP, etc.), the above strategies can be referred to while the reaction time may need to be adjusted.
After the reaction system is prepared, a drop of mineral oil can be added to cover the upper part of the reaction liquid to reduce aerosol pollution.
Preparation of complete amplification system using lyophilized microbeads: Pack optimized primers at the bottom of 0.2 ml EP tube and dry at 70-80°C for 1-2h. The dried 0.2 ml EP tube already contains dried amplification primers, and then add a lyophilized microbead to prepare the complete amplification system, which can be stored for a long time, without the need for cold chain transportation.
There is no detergent in the product. Adding Tween20 with a final concentration of 0.5% to the reaction system will accelerate the reaction speed for about 3min. Preparation method of Tween20 with 10× concentration: Tween20 is dissolved in ddH2O with a concentration of 5%, and the pH is adjusted to 8.8 with dilute NaOH solution (50mM). The solution is used as 10×, and 2.5μl is used for each microbead.
Under the condition of low-temperature storage, before opening the cap, the bottle shall be placed at room temperature to make the temperature of the lyophilized microbeads to be consistent with the room temperature, which can prevent moisture in the air from being absorbed by microbeads due to the difference in temperature.
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
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