Both DNA and RNA samples can be used for isothermal amplification with PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads. With freeze-drying technology, PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads can be transported and stored at room temperature.
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Cat. No.: IAMHMB-100 (for 100T)
Cat. No.: IAMHMB-1k (for 1,000T)
Cat. No.: IAMHMB-10k (for 10,000T)
PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads contain reaction buffer, HNB, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. After amplification, the positive samples are sky blue, while the negative samples are violet. The results can be directly observed by naked eyes without any other auxiliary equipment. PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for 6 months. PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads are so far one of the most robust systems for colorimetric isothermal amplification as it has a very good tolerance to pH and buffer salt.
The minimum shelf life is 6 months at 25°C and 2 years at -20°C. The product can be transported at room temperature. After the product is opened, the unused product should be sealed with sealing film or electrical tape to prevent performance degradation due to moisture. The product can also be vacuum packed in a 0.2 ml EP tube.
When used in other isothermal amplification reactions (such as CPA, SMAP, etc.), the above strategies can be referred to while the reaction time may need to be adjusted.
Preparation of complete amplification system using lyophilized microbeads: Pack optimized primers at the bottom of 0.2 ml EP tube and dry at 70 ~ 80 °C for 1 ~ 2h. The dried 0.2 ml EP tube already contains dried amplification primers, and then add a lyophilized microbead to prepare the complete amplification system, which can be stored for a long time, without the need for cold chain transportation.
Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.