By Journals
Science
Structure of the Activated Human Minor Spliceosome
Researchers investigated the structural basis of the activated human minor spliceosome using cryo-electron microscopy, providing important insights into pre-mRNA splicing mechanisms and spliceosome function.
During experimental validation, GoodView™ Nucleic Acid Stain was used for visualization of RT-PCR products following agarose gel electrophoresis.
Product Used: GoodView™ Nucleic Acid Stain
Publication: Bai, R., Wan, R., Wang, L., Xu, K., Zhang, Q., Lei, J., & Shi, Y. (2021). Structure of the activated human minor spliceosome. Science 371, eabg0879.
Cell
Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae
Researchers determined the structure of the post-catalytic spliceosome from Saccharomyces cerevisiae, providing important insights into the molecular mechanisms governing pre-mRNA splicing and spliceosome dynamics during the final stages of the splicing cycle.
To analyze RT-PCR products generated during the study, agarose gel electrophoresis was performed and nucleic acids were visualized using GoodView™ Nucleic Acid Stain.
Product Used: GoodView™ Nucleic Acid Stain
Publication: Bai, R., Yan, C., Wan, R., Lei, J., & Shi, Y. (2017). Structure of the post-catalytic spliceosome from Saccharomyces cerevisiae. Cell, 171(7), 1589-1598.
Cancer Cell
TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARα and Inhibition of p53-Mediated Senescence
Researchers investigated the role of TRIB3 in acute promyelocytic leukemia (APL) progression and demonstrated that TRIB3 promotes leukemogenesis through stabilization of the PML-RARα oncoprotein while suppressing p53-mediated cellular senescence.
To support mechanistic studies, multiple synthetic peptides and peptide variants, including S160, S490, S497, S160-scrambled, Pep2-con, Pep2-S160, and mutant Pep2-S160, were custom synthesized and utilized for functional characterization of protein interactions and signaling pathways.
Product Used: Custom Peptide Synthesis Service
Publication: Li, K., Wang, F., Cao, W. B., Lv, X. X., Hua, F., Cui, B., ... & Hu, Z. W. (2017). TRIB3 promotes APL progression through stabilization of the oncoprotein PML-RARα and inhibition of p53-mediated senescence. Cancer cell, 31(5), 697-710.
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Cell Metabolism
Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export
Researchers investigated the role of CD147 lysine methylation in non-small cell lung cancer (NSCLC) and demonstrated that di-methylation of CD147 at K234 promotes tumor progression by enhancing lactate export and metabolic adaptation.
To examine the functional significance of specific lysine residues, multiple CD147 mutants (K234R, K250R, K259R, and K261R) were generated using a Site-Directed Mutagenesis Kit from SBS Genetech. These engineered variants enabled precise analysis of residue-specific contributions to CD147 regulation and cancer progression.
Product Used: Site-Directed Mutagenesis Kit
Publication: Wang, K., Huang, W., Chen, R., Lin, P., Zhang, T., Ni, Y. F., ... & Chen, Z. N. (2023). Di-methylation of CD147-K234 promotes the progression of NSCLC by enhancing lactate export. Cell Metabolism, 35(6), 1084.
Links: View Site-Directed Mutagenesis Kit | Read Publication
Immunity
Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages
Researchers investigated the role of C/EBPβ in pulmonary fibrosis and demonstrated that promoting degradation of this transcription factor can alleviate lung fibrosis by restoring A20 activity in macrophages, highlighting a potential therapeutic strategy for fibrotic diseases.
To support mechanistic studies, multiple synthetic peptides, including Peptide A, B, C, D, and PA, were custom synthesized and utilized for experimental evaluation of protein function and regulatory pathways.
Product Used: Custom Peptide Synthesis Service
Publication: Liu, S. S., Lv, X. X., Liu, C., Qi, J., Li, Y. X., Wei, X. P., ... & Hu, Z. W. (2019). Targeting degradation of the transcription factor C/EBPβ reduces lung fibrosis by restoring activity of the ubiquitin-editing enzyme A20 in macrophages. Immunity, 51(3), 522-534.
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Nucleic Acids Research
A Thermodynamic Overview of Naturally Occurring Intramolecular DNA Quadruplexes
Researchers conducted a thermodynamic analysis of naturally occurring intramolecular DNA G-quadruplex structures, providing insights into their stability, folding behavior, and biological relevance in genomic regulation.
For experimental preparation, HPLC-purified oligonucleotides were synthesized and procured from SBS Genetech. These high-purity DNA oligonucleotides were used to ensure accurate thermodynamic measurements and reliable structural characterization of quadruplex formation.
Product Used: Custom DNA Oligonucleotide Synthesis (HPLC Purified Oligos)
Publication: A Thermodynamic Overview of Naturally Occurring Intramolecular DNA Quadruplexes. Nucleic Acids Research.
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Advanced Science
CDK5 Inhibition Abrogates TNBC Stem Cell Properties and Enhances Anti–PD-1 Therapy
Researchers investigated the role of CDK5 signaling in triple-negative breast cancer (TNBC) stem cell properties and demonstrated that CDK5 inhibition suppresses cancer stemness and enhances responsiveness to anti–PD-1 immunotherapy, highlighting a potential combinational therapeutic strategy.
To investigate PPARγ phosphorylation at Ser273, site-specific mutants (PPARγ-S273A and PPARγ-S273D) were generated using PCR-based site-directed mutagenesis from a Plenti-PPARγ plasmid template with a Muta-Direct Site-Directed Mutagenesis Kit from SBS Genetech.
Product Used: Site-Directed Mutagenesis Kit
Publication: Bei, Y., Cheng, N., Chen, T., Shu, Y., Yang, Y., Yang, N., ... & Shen, P. (2020). CDK5 inhibition abrogates TNBC stem‐cell property and enhances anti‐PD‐1 therapy. Advanced Science, 7(22), 2001417.
Links: View Site-Directed Mutagenesis Kit | Read Publication
Annals of the Rheumatic Diseases
MicroRNA-146a and Ets-1 Gene Polymorphisms in Ocular Behcet's Disease and Vogt–Koyanagi–Harada Syndrome
Researchers investigated the association between MicroRNA-146a and Ets-1 gene polymorphisms and the susceptibility to ocular Behcet’s disease and Vogt–Koyanagi–Harada (VKH) syndrome, aiming to better understand the genetic background of autoimmune uveitis disorders.
Restriction enzyme digestion products were analyzed by agarose gel electrophoresis (4% gels), and DNA fragments were visualized using GoodView™ Nucleic Acid Stain from SBS Genetech.
Product Used: GoodView™ Nucleic Acid Stain
Publication: Zhou, Q., Hou, S., Liang, L., Li, X., Tan, X., Wei, L., ... & Yang, P. (2014). MicroRNA-146a and Ets-1 gene polymorphisms in ocular Behcet's disease and Vogt–Koyanagi–Harada syndrome. Annals of the rheumatic diseases, 73(1), 170-176.
Nature Communications
Molecular Mechanisms Underlying Menthol Binding and Activation of TRPM8 Ion Channel
Researchers investigated the molecular basis of menthol binding and activation of the TRPM8 ion channel, providing insights into ligand recognition and channel gating mechanisms in temperature-sensing ion channels.
To study structure–function relationships, specific point mutations were generated using the Fast Mutagenesis Kit V2 from SBS Genetech, enabling targeted alteration of TRPM8 residues for functional analysis of channel activation.
Product Used: Site-Directed Mutagenesis Kit
Publication: Xu, L., Han, Y., Chen, X., Aierken, A., Wang, H., Lu, X., ... & Yang, F. (2020). Molecular mechanisms underlying menthol binding and activation of TRPM8 ion channel. Biophysical Journal, 118(3), 413a.
Links: View Site-Directed Mutagenesis Kit | Read Publication
Science Advances
Radiation-Induced Eosinophils Improve Cytotoxic T Lymphocyte Recruitment and Response to Immunotherapy
Researchers investigated how radiation-induced eosinophils modulate the tumor immune microenvironment and enhance cytotoxic T lymphocyte recruitment, thereby improving the efficacy of immunotherapy.
For antigen-specific T cell stimulation assays, single-cell suspensions were pulsed with hgp10025–33 peptide (KVPRNQDWL, immunograde quality), which was purchased from SBS Genetech, and used to assess antigen-specific cytotoxic T lymphocyte responses.
Product Used: Research-Grade Synthetic Peptides (Immunograde Peptides)
Publication: Cheng, J. N., Luo, W., Sun, C., Jin, Z., Zeng, X., Alexander, P. B., ... & Zhu, B. (2021). Radiation-induced eosinophils improve cytotoxic T lymphocyte recruitment and response to immunotherapy. Science advances, 7(5), eabc7609.
Links: View Custom Peptide Synthesis Service | Read Publication
Nature Protocols
Fluorescent Conjugated Polymer-Based FRET Technique for Detection of DNA Methylation in Cancer Cells
Researchers developed a fluorescent conjugated polymer-based FRET assay for sensitive detection of DNA methylation in cancer cells, providing a novel approach for epigenetic analysis and cancer diagnostics.
Following DNA amplification and assay processing, products were analyzed by agarose gel electrophoresis and visualized using GoodView™ DNA dye (HGV-1) from SBS Genetech.
Product Used: GoodView™ Nucleic Acid Stain
Publication: Feng, F., Liu, L., & Wang, S. (2010). Fluorescent conjugated polymer-based FRET technique for detection of DNA methylation of cancer cells. Nature protocols, 5(7), 1255-1264.
Clinical Cancer Research
Analysis of miR-195 and miR-497 Expression, Regulation and Role in Breast Cancer
Researchers investigated the expression patterns and regulatory roles of miR-195 and miR-497 in breast cancer, providing insights into their potential function as tumor-related microRNAs involved in cancer progression.
Restriction digestion products were separated by electrophoresis on 3% agarose gels, and nucleic acids were visualized using GoodView™ Nucleic Acid Stain from SBS Genetech.
Product Used: GoldView™ Nucleic Acid Stain
Publication: Li, D., Zhao, Y., Liu, C., Chen, X., Qi, Y., Jiang, Y., ... & Lai, L. (2011). Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer. Clinical cancer research, 17(7), 1722-1730.
Molecular Therapy
Non-invasive Oil-Based Method to Increase Topical Delivery of Nucleic Acids to Skin
Researchers developed a non-invasive oil-based delivery system to enhance the topical transport of nucleic acids into the skin, providing a potential strategy for improved transdermal gene delivery and dermatological applications.
To evaluate delivery efficiency, two synthetic peptides (Mgpe9 and TD-1, >98% purity) were custom synthesized by SBS Genetech and used as functional components in the formulation and experimental validation of nucleic acid skin penetration.
Product Used: Custom Peptide Synthesis (High-Purity Research Peptides)
Publication: Vij, M., Alam, S., Gupta, N., Gotherwal, V., Gautam, H., Ansari, K. M., ... & Ganguli, M. (2017). Non-invasive oil-based method to increase topical delivery of nucleic acids to skin. Molecular Therapy, 25(6), 1342-1352.
Links: View Custom Peptide Synthesis Service | Read Publication
Biomaterials
Recognition and Capture of Metastatic Hepatocellular Carcinoma Cells Using Aptamer-Conjugated Quantum Dots and Magnetic Particles
Researchers developed an aptamer-based system combined with quantum dots and magnetic particles for the recognition and capture of metastatic hepatocellular carcinoma cells, providing a potential strategy for circulating tumor cell detection and cancer diagnostics.
A FITC-labeled control aptamer (NK8, targeting Mycobacterium tuberculosis) was custom synthesized by SBS Genetech and used as a fluorescently labeled oligonucleotide control for evaluating binding specificity and assay performance.
Product Used: Custom Oligonucleotide Synthesis (FITC-Labeled Aptamers)
Publication: Wang, F. B., Rong, Y., Fang, M., Yuan, J. P., Peng, C. W., Liu, S. P., & Li, Y. (2013). Recognition and capture of metastatic hepatocellular carcinoma cells using aptamer-conjugated quantum dots and magnetic particles. Biomaterials, 34(15), 3816-3827.
Chemical Science
Structure and Effective Charge Characterization of Proteins by a Mobility Capillary Electrophoresis-Based Method
Researchers developed a mobility capillary electrophoresis-based method for characterizing protein structure and effective charge, providing a quantitative approach for studying protein physicochemical properties.
For method calibration and validation, synthetic peptides including bradykinin and VEALYL (pentapeptide) were custom synthesized by SBS Genetech and used as reference standards in electrophoretic mobility analysis.
Product Used: Custom Peptide Synthesis (Analytical Reference Peptides)
Publication: Zhang, W., Wu, H., Zhang, R., Fang, X., & Xu, W. (2019). Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method. Chemical science, 10(33), 7779-7787.
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Metabolic Engineering
Iteratively Improving Natamycin Production in Streptomyces gilvosporeus by a Large Operon-Reporter Based Strategy
Researchers developed an operon-reporter based strategy to iteratively improve natamycin production in Streptomyces gilvosporeus, providing a systematic approach for optimizing secondary metabolite biosynthesis in industrial microorganisms.
For transcript analysis, total RNA was extracted from S. gilvosporeus strains using a commercial RNA extraction kit from SBS Genetech, following the manufacturer’s protocol, to enable downstream gene expression profiling in engineered strains.
Product Used: Total RNA Extraction Kit
Publication: Wang, Y., Tao, Z., Zheng, H., Zhang, F., Long, Q., Deng, Z., & Tao, M. (2016). Iteratively improving natamycin production in Streptomyces gilvosporeus by a large operon-reporter based strategy. Metabolic Engineering, 38, 418-426.
Theranostics
G9a stimulates CRC growth by inducing p53 Lys373 dimethylation-dependent activation of Plk1
Researchers investigated the role of G9a-mediated epigenetic regulation in colorectal cancer (CRC) and demonstrated that G9a promotes tumor growth through p53 Lys373 dimethylation-dependent activation of Plk signaling pathways, revealing a key mechanism in cancer epigenetic control.
To study protein function and post-translational modification effects, human G9a and p53 mutants were generated using the Muta-direct Site-Directed Mutagenesis Kit from SBS Genetech, enabling precise construction of plasmid variants for mechanistic analysis.
Product Used: Site-Directed Mutagenesis Kit
Publication: Zhang, J., Wang, Y., Shen, Y., He, P., Ding, J., & Chen, Y. (2018). G9a stimulates CRC growth by inducing p53 Lys373 dimethylation-dependent activation of Plk1. Theranostics, 8(10), 2884.
Links: View Site-Directed Mutagenesis Kit | Read Publication
Journal of the National Cancer Institute
The Role of Polymeric Immunoglobulin Receptor in Inflammation-Induced Tumor Metastasis of Human Hepatocellular Carcinoma
Researchers investigated the role of the polymeric immunoglobulin receptor (pIgR) in inflammation-induced tumor metastasis in hepatocellular carcinoma, providing insights into how inflammatory signaling contributes to cancer progression and metastatic behavior.
To evaluate the functional role of pIgR, human pIgR mutants were generated using the Muta-direct Site-Directed Mutagenesis Kit from SBS Genetech, enabling precise construction of mutant plasmids for mechanistic studies of protein function in tumor metastasis.
Product Used: Site-Directed Mutagenesis Kit
Publication: Ai, J., Tang, Q., Wu, Y., Xu, Y., Feng, T., Zhou, R., ... & Geng, M. (2011). The role of polymeric immunoglobulin receptor in inflammation-induced tumor metastasis of human hepatocellular carcinoma. Journal of the National Cancer Institute, 103(22), 1696-1712.
Links: View Site-Directed Mutagenesis Kit | Read Publication
Cancer Letters
Disruption of the EGFR–SOSTM1 Interaction by a Stapled Peptide Suppresses Lung Cancer via Activating Autophagy and Inhibiting EGFR Signaling
Researchers investigated a stapled peptide-based strategy to disrupt the EGFR–SOSTM1 protein–protein interaction, demonstrating suppression of lung cancer progression through activation of autophagy and inhibition of EGFR signaling pathways.
Chimeric peptides, SAH-EJ stapled peptides, and their fluorescently labeled analogues were custom synthesized by SBS Genetech and used for functional evaluation of protein–protein interaction disruption and signaling pathway modulation.
Product Used: Custom Peptide Synthesis (Stapled & Fluorescently Labeled Peptides)
Publication: Yu, J. J., Zhou, D. D., Cui, B., Zhang, C., Tan, F. W., Chang, S., ... & Hua, F. (2020). Disruption of the EGFR-SQSTM1 interaction by a stapled peptide suppresses lung cancer via activating autophagy and inhibiting EGFR signaling. Cancer Letters, 474, 23-35.
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