RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)
$1,276.00 - $8,294.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: RMIF4-96 (for 96T)
Cat. No.: RMIF4-960 (for 960T)
Description
The RPA Multiplex Fluorescent Lyophilized Kit (Gen-4) is designed for rapid, multiplex nucleic acid amplification using recombinase polymerase amplification (RPA). Its optimized reaction system supports the simultaneous amplification of 2–4 targets in approximately 10–20 minutes, making it suitable for developing rapid molecular detection assays.
The kit incorporates reverse transcription (RT), enabling the detection of RNA targets through reverse transcription followed by RPA amplification. Its lyophilized formulation facilitates reagent handling and transportation, while the flexible assay configuration supports both fluorescence-based detection and compatible lateral flow assays.
With a minimum detection limit of 400 copies/mL demonstrated across multiple validated targets under specific assay conditions, this kit provides a versatile foundation for multiplex molecular assay development.
Key Features
Multiplex Amplification
Supports simultaneous amplification of 2–4 nucleic acid targets in a single reaction system, depending on assay design and validation.
DNA and RNA Target Detection
The integrated reverse transcription capability enables RNA target detection through RT-RPA, in addition to DNA target amplification.
Rapid Amplification
The amplification reaction can be completed in approximately 10–20 minutes. Total assay time may vary depending on sample preparation and the selected detection method.
Lyophilized Formulation
The freeze-dried reagent format supports convenient transportation and storage under specified conditions. Reagents are supplied in separate lyophilized containers and require preparation before use.
Flexible Detection Options
Suitable for fluorescence-based detection and compatible lateral flow assays when the appropriate assay configuration, primers, labels, and detection components are used.
Validated Analytical Sensitivity
A minimum detection limit of 400 copies/mL has been demonstrated across multiple validated targets. Actual performance depends on the target, sample matrix, assay configuration, and testing conditions.
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Frequently Asked Questions
1. Can this kit be used for both DNA and RNA detection?
Yes. The kit includes reverse transcription capability. DNA targets can be amplified by RPA, while RNA targets are detected through reverse transcription followed by RPA amplification. Assay performance should be validated for each target.
2. How many targets can be amplified simultaneously?
The kit supports multiplex amplification of 2–4 targets. The actual number of targets that can be detected reliably depends on primer design, target compatibility, and assay optimization.
3. What detection methods are supported?
The kit is designed for fluorescence-based detection and can also be used with compatible lateral flow assays. The required primers, labels, reaction setup, and detection components may differ between assay formats. Please contact our technical team to confirm compatibility with your intended application.
4. What is the detection limit?
A minimum detection limit of 400 copies/mL has been demonstrated across multiple validated targets under specific assay conditions. This value should not be interpreted as a universal detection limit for every target or sample type.
5. How long does the amplification reaction take?
The amplification reaction typically takes approximately 10–20 minutes. Sample preparation, reverse transcription where applicable, and signal detection may add to the total assay time.
6. Are the reagents ready to use directly from the package?
The reagents are supplied in separate lyophilized containers rather than pre-dispensed reaction tubes. Preparation and reaction setup are required before use. Please refer to the product instructions for the recommended reconstitution procedure and reaction setup.
7. How should the kit be stored and transported?
The specified storage and transportation conditions are 2–28°C in a dry, light-protected environment. The stated shelf life is one year under the specified conditions. Please refer to the product label and instructions for the applicable handling requirements.
8. Can this kit be used to develop custom molecular detection assays?
The kit is intended to support multiplex molecular assay development. Target-specific primer design, optimization, and analytical validation are recommended before applying the assay to new targets or sample types.
Need Technical Assistance?
For information about primer compatibility, multiplex assay development, lateral flow detection, or bulk orders, please contact our technical team.
One More Thing
Based on our leading RPA technology, we have developed a series of reagent kits to enrich the field of POCT.
| No. | Name | Method | Sample | Process | Lowest Detection Limit (LOD) |
|---|---|---|---|---|---|
| 1 | Tuberculosis Complex Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Tongue swab | Nucleic acid release/direct amplification | 500 copies/mL |
2 | Type A Influenza Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Throat and nasal swab | Nucleic acid release/direct amplification | 500 copies/mL |
3 | Type B Influenza Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Throat and nasal swab | Nucleic acid release/direct amplification | 500 copies/mL |
4 | Type A/B Influenza Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Throat and nasal swab | Nucleic acid release/direct amplification | 500 copies/mL |
| 5 | Mycoplasma pneumoniae Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Throat and nasal swab | Nucleic acid release/direct amplification | 500 copies/mL |
6 | Cytomegalovirus A/B Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Throat and nasal swab | Nucleic acid release/direct amplification | 500 copies/mL |
7 | Chlamydia trachomatis/Neisseria gonorrhoeae Combined Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Vaginal swab | Nucleic acid release/direct amplification | 500 copies/mL |
8 | Human Immunodeficiency Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Whole blood | Nucleic acid release/direct amplification | 500 copies/mL |
9 | Chlamydia trachomatis Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Vaginal swab | Nucleic acid release/direct amplification | 500 copies/mL |
10 | Neisseria gonorrhoeae Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Vaginal swab | Nucleic acid release/direct amplification | 500 copies/mL |
11 | Human Papillomavirus 15 High-Risk Type Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Vaginal swab | Nucleic acid release/direct amplification | 500 copies/mL |
12 | African Swine Fever Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Oral, nasal, and eye swab | Nucleic acid release/direct amplification | 500 copies/mL |
13 | Rabies Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Oral, nasal, and eye swab, whole blood | Nucleic acid release/direct amplification | 500 copies/mL |
| 14 | Brucella Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Oral, nasal, and eye swab | Nucleic acid release/direct amplification | 500 copies/mL |
15 | Canine Distemper Virus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Oral, nasal, and eye swab | Nucleic acid release/direct amplification | 500 copies/mL |
| 16 | Canine Parvovirus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Oral, nasal, and eye swab | Nucleic acid release/direct amplification | 500 copies/mL |
| 17 | Canine Adenovirus Rapid Amplification Kit (with internal reference) | Real-time fluorescence/paper strip | Oral, nasal, and eye swab | Nucleic acid release/direct amplification | 500 copies/mL |
Related:
- Gen-1: RPA Basic Kit (Gen-1), RPA RT Basic Kit (Gen-1), RPA Fluorescent Kit (Gen-1), RPA RT Fluorescent Kit (Gen-1)
- Gen-2: RPA Basic Kit (Gen-2), RPA RT Basic Kit (Gen-2), RPA Fluorescent Kit (Gen-2), RPA RT Fluorescent Kit (Gen-2)
- Gen-3: RPA Basic Kit (Gen-3), RPA RT Basic Kit (Gen-3), RPA Fluorescent Kit (Gen-3), RPA RT Fluorescent Kit (Gen-3)
- Gen-4: RPA Multiplex Basic Lyophilized Kit (Gen-4), RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)
- Lateral Flow Assay for RPA
Only for research and not intended for treatment of humans or animals
