Lateral Flow Assay for RPA
$395.00 - $695.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: LFAR-50 (for 50T)
Cat. No.: LFAR-100 (for 100T)
Description
The Lateral Flow Assay for RPA is designed for rapid, visual detection of nucleic acid amplification products using lateral flow test strips.
The assay uses a dual-label detection strategy. Amplification products are generated using primers labeled with biotin and FITC or 6-FAM, allowing the labeled products to be detected by the corresponding test strips.
The workflow is simple: perform nucleic acid amplification, apply the prepared reaction product to the test strip, and visually interpret the result. No dedicated strip-reading instrument is required.
Compatible with appropriately labeled products generated by RPA and other suitable amplification methods, this assay provides a convenient endpoint detection option for molecular assay development.
Key Features
Rapid Visual Detection
Detect compatible nucleic acid amplification products through visible test and control lines, without requiring a dedicated strip-reading instrument.
Dual-Label Recognition
Designed for amplification products incorporating biotin and FITC or 6-FAM labels. Both labels must be incorporated into the same amplification product for detection.
Compatible with Isothermal Amplification
Suitable for RPA and RAA amplification products when the required labeling strategy and reaction conditions are met. Compatibility with other amplification methods should be confirmed for the intended assay.
Simple Endpoint Readout
The test strip provides a visual endpoint result, making it suitable for research workflows that require rapid nucleic acid detection without gel electrophoresis.
Single-Use Format
Each strip is intended for one sample and one-time use.
Specifications
Parameter
| Specification
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Important: Compatible amplification primers, appropriate labeling, and validated reaction preparation are required. The lateral flow assay does not itself perform nucleic acid amplification.
Operating Steps
- According to the number of samples to be tested, take out the corresponding number of test strips and mark them on the absorbent pad. Each test strip can only be used once for a single sample.
- When the amplification product volume is 50–100 µL, nucleic acid products can be directly tested in a 200 µL PCR reaction tube. If the product volume is less than 50 µL, add ultrapure water to the PCR tube to make up the volume to 50 µL, mix thoroughly, and then proceed with detection.
- After PCR, RPA, or RAA reaction is completed, open the PCR reaction tube and insert the test strip with the binding pad end (arrow end) into the PCR tube (Figure 1). The liquid level must not exceed the upper edge of the binding pad. Wait until the interpretation area is fully saturated (approximately 1–2 minutes; in colder environments such as winter, absorption speed will be slower, and saturation time will be longer). Once the control line (C line) appears, the test strip can be removed.
- For RPA or RAA reactions, dilution is required before inserting the test strip.
- Observe the results within 10 minutes after the control line (C line) appears. Results read after 10 minutes are invalid.
- Record the test results, then seal and discard the test strip in a safe location.
Results
Positive (+):
A blue band appears at the control line (C line) and a red band appears at the test line (T line). A positive result indicates that the sample contains the target nucleic acid fragment, and its quantity is greater than the minimum detection limit of the test strip.
- When the concentration of the target nucleic acid product is low, the C line appears blue and the T line appears light red or even pale pink. This result should still be interpreted as positive.
- When the concentration of the target nucleic acid product is high, both the C line and the T line appear red. This result should also be interpreted as positive.
Negative (–):
A blue band appears at the control line (C line), while no band appears at the test line (T line). A negative result indicates that the sample does not contain the target nucleic acid fragment, or its quantity is below the minimum detection limit of the test strip.
Invalid:
No bands appear at either the control line (C line) or the test line (T line). This suggests that the test strip or amplification reagents may be damaged, expired, or that an operational error has occurred. In this case, carefully read the instructions, repeat amplification and testing. If the problem persists, immediately discontinue use of products from the same batch and contact your local supplier.
Frequently Asked Questions
1. Does this product amplify nucleic acids?
No. This product detects nucleic acid amplification products. A separate amplification reaction must be performed before using the strip.
2. What labels are required for detection?
The amplification product must incorporate biotin and FITC or 6-FAM. Both labels must be present on the same product for the intended detection mechanism to work.
3. Can this product be used with any RPA kit?
Compatibility depends on the labeling strategy, amplification conditions, and product configuration. The primers must be appropriately labeled, and the resulting amplification products must be compatible with the strip.
4. Can the strip be used with RAA or PCR products?
The current protocol describes use with RPA, RAA, and PCR amplification products. However, successful detection depends on appropriate labeling and validated assay conditions. Confirm compatibility before using a new amplification system.
5. Is a dedicated reader required?
No dedicated strip-reading instrument is required. Results are interpreted visually by observing the control and test lines.
6. How long does the test take?
The strip typically develops within approximately 1–2 minutes. Read the result within 10 minutes after the control line appears, according to the product instructions.
7. Does the amplification product need to be diluted?
Yes. The current protocol specifies dilution for RPA and RAA products before applying them to the strip. Follow the validated procedure for the relevant amplification system.
8. How should the strips be stored?
Store the strips at 4–30°C in a dry, light-protected environment and use them within the stated shelf life.
Precautions
- For research use only. This product is intended solely for scientific research. Before use, carefully read the instructions and strictly follow the operating procedures. Failure to comply may result in incorrect results.
- Storage and validity. Store the product according to the requirements stated in the instructions, under suitable environmental conditions and temperatures, and use it within the validity period. Improper storage or use of expired products may lead to erroneous results.
- Use promptly after opening. Once the package is opened, use the test strips as soon as possible to avoid moisture absorption, which may affect test performance.
- Environmental and operator factors. Insufficient lighting in the testing environment or operator color vision deficiencies may also cause incorrect results.
- Disposal and single-use notice. After use, promptly place the test strip into a sealed bag and dispose of it appropriately. This product is designed for one-time use only and must not be reused.
Storage
Store in a light-protected and dry place, at a temperature of 4~30°C, with a shelf life of 12 months.
Instruction: Protocol
Need Technical Assistance?
For information about primer compatibility, multiplex assay development, lateral flow detection, or bulk orders, please contact our technical team.
Related:
- Gen-1: RPA Basic Kit (Gen-1), RPA RT Basic Kit (Gen-1), RPA Fluorescent Kit (Gen-1), RPA RT Fluorescent Kit (Gen-1)
- Gen-2: RPA Basic Kit (Gen-2), RPA RT Basic Kit (Gen-2), RPA Fluorescent Kit (Gen-2), RPA RT Fluorescent Kit (Gen-2)
- Gen-3: RPA Basic Kit (Gen-3), RPA RT Basic Kit (Gen-3), RPA Fluorescent Kit (Gen-3), RPA RT Fluorescent Kit (Gen-3)
- Gen-4: RPA Multiplex Basic Lyophilized Kit (Gen-4), RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)
- Lateral Flow Assay for RPA
SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.
