CRISPR Cas12a RNA Detection Kit (Two-Step) (Test Strip)
$935.00 - $3,270.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: C12ARTP-96 (for 96T)
Cat. No.: C12ARTP-480 (for 480T)
Description
Cas12a and crRNA form a functional complex that "slides" along the target nucleic acid sequence. Upon successful pairing, Cas12a is specifically activated and cleaves surrounding reporter molecules. This kit combines isothermal amplification technology with Cas12a, offering high sensitivity, high specificity, and a high signal-to-noise ratio. It is widely used in molecular diagnostics for the rapid and accurate detection of pathogens.
Kit Components
| No. 1 | Item Reaction Buffer (2X) | Size 1000 μl |
2 | Core Mix (4X) | 500 μl |
3 | Positive Control (10X) (primer and RNA template included) | 30 μl |
4 | Cleavage Buffer (10X) | 240 μl |
5 | Cas12a Protein (10 μM) | 20 μl |
6 | crRNA for Positive Control (20X) | 20 μl |
7 | Reporter (4 μM) | 80 μl |
8 | Starter (10X) | 200 μl |
9 | Diluent | 8000 μl |
Additional Required Materials
- Nucleic acid test strips (FAM-Biotin product, we recommend Lateral Flow Assay for CRISPR SHERLOCK)
- Isothermal equipment (e.g., metal bath, water bath, PCR instrument)
- Pipettes
- Nuclease-free water
- Target sequence-specific primers (for isothermal amplification)
- Note: The primers must include the T7 promoter: 5’-TAATACGACTCACTATAG-3’
- crRNA/gRNA: Forms a functional complex with LbaCas12a and is specifically activated by the target sequence.
- LbaCas12a crRNA scaffold sequence: 5’-UAAUUUCUACUAAGUGUAGAU-3’
Related:
- CRISPR Cas12a DNA Detection Kit (One-Step)
- CRISPR Cas12a DNA Detection Kit (Two-Step)
- CRISPR Cas12a DNA Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a DNA Detection Kit (One-Step)
- CRISPR Cas13a DNA Detection Kit (Two-Step)
- CRISPR Cas12a RNA Detection Kit (One-Step)
- CRISPR Cas12a RNA Detection Kit (Two-Step)
- CRISPR Cas12a RNA Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a RNA Detection Kit (One-Step)
- CRISPR Cas13a RNA Detection Kit (Two-Step)
- CRISPR Cas13a RNA Detection Kit (Two-Step) (Test Strip)
- Lateral Flow Assay for CRISPR SHERLOCK
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