Heat-Labile Uracil DNA Glycosylase (HL-UDG, 1 U/μl)
500.00 - 3,250.00
Heat-Labile Uracil-DNA Glycosylase is a recombinant protein expressed by E. coli which is originally from psychrotrophic marine bacteria. Depending on how the DNA is prepared, HL-UDG can be used to achieve general, site-specific, or strand-specific U-DNA cleavage. This enzyme shows lower thermostability and is, therefore, easier to inactivate.
Heat-Labile Uracil DNA Glycosylase can effectively hydrolyze uracil-glycosidic bonds in single- or double-stranded DNA, excising uracil and creating alkali-sensitive abasic sites in the DNA, which can be hydrolyzed by endonuclease, heat, or alkali treatment. The enzyme has no activity to RNA and is mainly used to prevent contamination in the PCR reaction system. The uracil DNA glycosylase from E.coli is relatively heat-resistant, and a small amount of uracil DNA glycosylase activity remains after being treated at 95°C for 10 min, which leads to the degradation of PCR products containing dU base. While the HL-UDG from psychrophilic marine bacteria is completely inactivated at 50°C for 5 min. Before PCR amplification, HL-UDG is added to the PCR reaction system, and the contamination in the system could be eliminated at 25°C for 10 min. HL-UDG will then be inactivated in the first step of denaturation (at 94°C) of the PCR cycle, preventing the degradation of the synthesized PCR products containing dU.
One unit is defined as the amount of enzyme required to catalyze the release of 60 pmol uracil from the double-stranded DNA containing uracil at 37°C per minute.
HL-UDG is compatible with most PCR reaction buffers, but its activity is inhibited at high ion concentrations (> 100 mm).
20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, , 50% Glycerol, 0.1% (w/v) Triton X-100, pH 7.5.
The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.
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