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Cat. No.: CmeCas12a-100 (for 100pmol)

Cat. No.: CmeCas12a-1k (for 1000pmol)

Description

CmeCas12a is derived from a compost metagenome and is a thermostable, crRNA‑guided DNA endonuclease. It exhibits robust catalytic activity across a broad temperature range (37–56 °C), with an optimal reaction temperature of 52 °C. CmeCas12a preferentially recognizes the PAM sequence TTTV (V = A/C/G). For double‑stranded DNA (dsDNA) targets, efficient recognition and cleavage require the presence of a PAM site, whereas single‑stranded DNA (ssDNA) targets can be cleaved without PAM dependence.

Moreover, both dsDNA and ssDNA targets can activate the trans‑cleavage activity of CmeCas12a (also known as collateral or auxiliary cleavage). Once CmeCas12a binds with crRNA and the target DNA to form a ternary complex, the enzyme becomes activated and nonspecifically cleaves surrounding ssDNA reporters in the reaction system.

Because of these properties, CmeCas12a can be used not only for specific in vitro cleavage of dsDNA but also for rapid detection of target nucleic acids.

Storage