SpCas9 is a DNA endonuclease derived from Streptococcus pyogenes, guided by crRNA and tracrRNA (or a single sgRNA). It specifically recognizes and cleaves double-stranded DNA (dsDNA) targets in a PAM (NGG)-dependent manner. Cas9 introduces a double-strand break at a site located 3 bp upstream of the PAM sequence. In addition, SpCas9 can specifically cleave single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA) in the presence of a DNA PAMmer sequence. SpCas9 is also widely used in vitro for targeted DNA cleavage and cloning applications.

This product is a glycerol-free, lyophilized SpCas9 nuclease that can be stored and transported at ambient temperature. It is available in multiple lyophilized formats (powder or bead) to meet the requirements of different downstream applications.

• SpCas9 Nuclease (Lyophilized) + Cas Reconstitution Solution: Stable at room temperature for storage and transport. After reconstitution, store at –20 °C and avoid repeated freeze–thaw cycles.
• 10× Buffer for Cas9: Store at –20 °C; transport at 4 °C.

Notes

• To prevent RNase contamination, keep the work area clean and tidy. Always wear clean gloves and a mask during operation, and ensure that all consumables (pipette tips, centrifuge tubes, etc.) are RNase-free.
• This product contains only the Cas nuclease. Target DNA and crRNA required for the CRISPR reaction system must be prepared separately. For the 10× Cas9 buffer, it is recommended to source from TOLOBIO.
• After reconstitution, SpCas9 should be handled on ice when preparing reaction mixtures. Immediately return the enzyme to –20 °C after use. At –20 °C, the reconstituted enzyme is stable for up to one month.
• For research use only.

SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.

Related: 

• Synthetic sgRNAs
• Cas12a Nuclease (Lyophilized)
• Cas12b Nuclease (Lyophilized)
• Cas13a Nuclease (Lyophilized)
• Cas14a1 Nuclease (Lyophilized)
• SpCas9 Nuclease (Lyophilized)
• SuCas12a2 Nuclease (Lyophilized)
• Cas9 Nuclease (with NLS)
• Cas9 HF-NLS (SpCas9 HF-NLS)
• CRISPR-dCas9 Nuclease
• dCas9 NLS
• spCas9
• SpdCas9
• spCas9-NG
• SpRYCas9
• Cas9 Nickase (H840A) NLS
• Cas9 Nickase (D10A) NLS
• TsCas12a
• OsCas12a
• HkCas12a
• BoCas12a
• AsCas12a
• FnCas12a
• LbCas12a (Cpf1) Nuclease
• Enhanced LbaCas12a (Cpf1)
• Lb5Cas12a (Cpf1) Nuclease
• SuCas12a2 Nuclease
• AaCas12b
• AacCas12b
• AapCas12b
• BrCas12b
• LwCas13a
• LbuCas13a
• LtrCas13a (C2c2) Nuclease
• LwaCas13a (C2c2) Nuclease
• EiCsm6
• PfAgo
• TtAgo
• T7 Endonuclease I

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