T7 endonuclease I recognizes and cleaves incompletely paired DNA, cross-structured DNA, Holliday structured or cross-structured DNA, heteroduplex DNA, or double-stranded DNA containing cleavage at a slower rate. T7 endonuclease I cleaves the first, second, or third phosphodiester at the 5'-end of the mismatched base. This product is a high purity protein obtained by cloning and recombinant expression of T7 endonuclease I gene, which has no other endonuclease or exonuclease contamination.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: T7EN1-250 (for 250U)
Cat. No.: T7EN1-2500 (for 2,500U)
In a 50 μL reaction system, at 37°C for 1 h, the amount of enzyme required to convert more than 90% of the pUC(AT)* of a 1 ug supercoiled cross-shaped structure into more than 90% of the linear structure is defined as an active unit.
Detection of mutant caused by gene mutation, SNP, TALEN, or CRISPR/Cas9
Recognition of mismatched DNA
Decomposition of four-way junction DNA or Branched DNA
Detection or cutting of heterodimer DNA
Shot-gun cloning by random cutting of linear DNA
1x T7 Endonuclease I Buffer
50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.9 at 25°C).
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5.
The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.
T7 endonuclease I is an enzyme with substrate structure selectivity. It acts on different DNA substrates with different activities. To cut a specific substrate, the amount of enzyme and reaction time must be carefully controlled.
When the reaction temperature exceeds 42°C, the non-specific nuclease activity will be increased.
Only for research and not intended for treatment of humans or animals
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