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Cas14a1 Nuclease (Lyophilized)

Cas14a1 Nuclease (Lyophilized)

$425.00 - $1,275.00
$1,700.00
Cas14a1 is a type of endonuclease that acts as a restriction enzyme, specifically binding and cleaving target single-stranded DNA (ssDNA) under the guidance of tracrRNA:crRNA (or sgRNA), and it does not require a PAM site. The lyophilized version of Cas14a1 can be transported at room temperature, saving the high cost of dry ice transportation.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: Cas14aLyo-100 (for 100pmol)

Cat. No.: Cas14aLyo-1k (for 1000pmol)

 

Description

Cas14a1 is a type of endonuclease that acts as a restriction enzyme, specifically binding and cleaving target single-stranded DNA (ssDNA) under the guidance of tracrRNA:crRNA (or sgRNA), and it does not require a PAM site.

Compared to other Cas proteins, Cas14 proteins generally have a smaller molecular weight (400-700aa). Similar to Cas12, Cas14a1 can bind to target nucleic acids and activate its ssDNA endonuclease activity, making it applicable for molecular detection of target nucleic acids. Unlike Cas12, Cas14a1 can only bind to ssDNA targets. Therefore, target nucleic acids that have undergone amplification enrichment need to be treated with T7 endonuclease, and one of the amplification primers needs to be phosphorothioate-modified to ensure that T7 endonuclease cleaves only one strand, leaving the ssDNA target strand for Cas14a1-mediated molecular detection.

The lyophilized version of Cas14a1 can be transported at room temperature, saving the high cost of dry ice transportation.

 

Transportation

Both Cas14a1 and 10×Buffer can be transported at room temperature.

 

Storage

The minimum shelf life is 18 months at 2-28°C for Cas14a1 and 1 year at -20°C for 10×Buffer.

 

Instruction: Protocol

 

SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.

 

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Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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