Cas12a (Cpf1) belongs to class II and type VI CRISPR system effector proteins, and is an endonuclease that binds to and cleavages specific sites of target DNA under the guidance of single-stranded guide RNA. It has been widely used in gene editing of microorganisms, plants, and animals, and has a great practical application value in molecular diagnosis. The lyophilized version of Cas12a can be transported at room temperature, saving the high cost of dry ice transportation.
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Cat. No.: Cpf1Lyo-100 (for 100pmol)
Cat. No.: Cpf1Lyo-2k (for 2000pmol)
Cas12a recognizes and cleaves target double-stranded DNA (dsDNA), and its collateral cleavage activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA). CRISPR/Cas12a can be used to detect DNA templates and amplify signals by designing DNA probes labeled with fluorescent groups or other markers at both ends. Common detection methods include real-time fluorescence and colloidal gold chromatography. GRNA specifically recognizes the target region, the CRISPR/Cas12a system is equivalent to a "secondary screening" of target DNA or DNA amplification products, so it has high specificity.
Lyophilized powder dissolution: Add enzyme-free H2O to dissolve, and it is recommended to dissolve it when it will be used. It can be stored at -20°C for 12 months. The recommended final concentration is 2pmol/μl.
Both Cas12a and 10×Buffer can be transported at room temperature.
The minimum shelf life is 1 year at room temperature for Cas12a and 1 year at -20°C for 10×Buffer.