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Cat. No.: OsCas12a-100 (for 100pmol)

Cat. No.: OsCas12a-1k (for 1000pmol)

Description

Cas12a nuclease (formerly known as Cpf1) is an RNA-guided endonuclease that specifically cleaves target double-stranded DNA in the presence of a PAM sequence. Unlike Cas9, Cas12a has several distinct features: (1) It requires only a single crRNA, is smaller in size, and is more easily delivered into cells; (2) It produces sticky ends upon cleavage, which are more conducive to precise genome editing; (3) Its cleavage site is distant from its recognition site, allowing for the possibility of consecutive editing; (4) It recognizes different PAM sequences compared to Cas9, offering alternative editing site options. Different Cas12a proteins require different PAM sequences for target recognition, with some recognizing TTN sites and others recognizing TTTN sites.

Storage

Store at -20°C and transport at ≤ 0°C.

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Related: 

• Synthetic sgRNAs
• Cas12a Nuclease (Lyophilized)
• Cas12b Nuclease (Lyophilized)
• Cas13a Nuclease (Lyophilized)
• Cas14a1 Nuclease (Lyophilized)
• Cas9 Nuclease (with NLS)
• Cas9 HF-NLS (SpCas9 HF-NLS)
• CRISPR-dCas9 Nuclease
• dCas9 NLS
• spCas9
• SpdCas9
• spCas9-NG
• SpRYCas9
• Cas9 Nickase (H840A) NLS
• Cas9 Nickase (D10A) NLS
• TsCas12a
• OsCas12a
• HkCas12a
• BoCas12a
• AsCas12a
• FnCas12a
• LbCas12a (Cpf1) Nuclease
• Enhanced LbaCas12a (Cpf1)
• Lb5Cas12a (Cpf1) Nuclease
• SuCas12a2 Nuclease
• AaCas12b
• AacCas12b
• AapCas12b
• BrCas12b
• LwCas13a
• LtrCas13a (C2c2) Nuclease
• LwaCas13a (C2c2) Nuclease
• EiCsm6
• PfAgo
• TtAgo
• T7 Endonuclease I