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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
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PfAgo

PfAgo

$1,600.00 - $4,800.00
PfAgo, derived from recombinant expression in Escherichia coli, is a programmable DNA endonuclease. Under the guidance of a short 16-18 nucleotides of 5'-phosphorylated single-stranded DNA (gDNA), the corresponding specific sequence on the substrate is digested, and a break is introduced in the phosphodiester main chain of the complementary substrate sequence. 
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Cat. No.: PFAGO-100 (for 100 μl)

Cat. No.: PFAGO-500 (for 500 μl)

 

Description

PfAgo, derived from recombinant expression in Escherichia coli, is a programmable DNA endonuclease. Under the guidance of a short 16-18 nucleotides of 5'-phosphorylated single-stranded DNA (gDNA), the corresponding specific sequence on the substrate is digested, and a break is introduced in the phosphodiester main chain of the complementary substrate sequence. 

The advantage of PfAgo is that the guiding design and target sequence selection are not limited by PAM, and it has high activity on single-stranded DNA and most double-stranded DNA substrates. In the presence of Mg2+ or Mn2+ metal ions, the enzyme obtains RNase H like activity and cuts the substrate at the base complementary to the 10th and 11th bases of the DNA guide chain.

 

Components

  • PfAgo (1 µM): 100 μl
  • 10 × Reaction buffer: 50 μl
  • MnCl2 (25 mM): 20 μl

 

Features

  • High sensitivity
  • Good specificity
  • High flux

 

Applications

  • Target nucleic acid molecular detection
  • Gene editing
  • Directed digestion

 

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.

 

Related: 

  • Synthetic sgRNAs
  • Cas12a Nuclease (Lyophilized)
  • Cas12b Nuclease (Lyophilized)
  • Cas13a Nuclease (Lyophilized)
  • Cas14a1 Nuclease (Lyophilized)
  • Cas9 Nuclease (with NLS)
  • Cas9 HF-NLS (SpCas9 HF-NLS)
  • CRISPR-dCas9 Nuclease
  • dCas9 NLS
  • spCas9
  • SpdCas9
  • spCas9-NG
  • SpRYCas9
  • Cas9 Nickase (H840A) NLS
  • Cas9 Nickase (D10A) NLS
  • TsCas12a
  • OsCas12a
  • HkCas12a
  • BoCas12a
  • AsCas12a
  • FnCas12a
  • LbCas12a (Cpf1) Nuclease
  • Enhanced LbaCas12a (Cpf1)
  • Lb5Cas12a (Cpf1) Nuclease
  • SuCas12a2 Nuclease
  • AaCas12b
  • AacCas12b
  • AapCas12b
  • BrCas12b
  • LwCas13a
  • LtrCas13a (C2c2) Nuclease
  • LwaCas13a (C2c2) Nuclease
  • EiCsm6
  • PfAgo
  • TtAgo
  • T7 Endonuclease I

 

 

 
 
Only for research and not intended for treatment of humans or animals
 
 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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