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PfAgo

PfAgo

$1,600.00 - $4,800.00
PfAgo, derived from recombinant expression in Escherichia coli, is a programmable DNA endonuclease. Under the guidance of a short 16-18 nucleotides of 5'-phosphorylated single-stranded DNA (gDNA), the corresponding specific sequence on the substrate is digested, and a break is introduced in the phosphodiester main chain of the complementary substrate sequence. 
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: PFAGO-100 (for 100 μl)

Cat. No.: PFAGO-500 (for 500 μl)

 

 

Description

PfAgo is an Argonaute protein derived from the hyperthermophilic archaeon Pyrococcus furiosus, exhibiting programmable endonuclease activity. Guided by a 5'-phosphorylated single-stranded DNA (gDNA), it specifically recognizes and cleaves complementary target DNA sequences. The guide DNA (gDNA) typically consists of a 16–18 nt, 5'-phosphorylated single-stranded oligonucleotide. The cleavage site is precisely located between the 10th and 11th nucleotides from the 5' end of the complementary guide sequence.

Lyophilized formulation available for ambient-temperature transport to cut shipping expenses. See PfAgo (Lyophilized) for more information.

 

Components

  • PfAgo
  • PfAgo Reaction Buffer
  • MnCl2

 

Key Features of PfAgo

  • Thermophilic Activity (Optimal Temperature ~95°C)

    Exhibits exceptional thermostability with peak catalytic activity at around 95°C, making it ideal for high-temperature molecular workflows.

  • PAM-Independent Cleavage (Distinct from CRISPR Systems)

    Requires no Protospacer Adjacent Motif (PAM) sequences, granting total flexibility in target site selection across the entire genome.

  • DNA-Guided Precision Targeting

    Utilizes cost-effective, highly stable 5'-phosphorylated short single-stranded DNA (gDNA) guides for flawless target recognition.

  • Dual-Substrate Cleavage Capability (dsDNA & ssDNA)

    Capable of cleaving both double-stranded and single-stranded DNA targets with high endonuclease efficiency.

  • Versatile Application Scenarios (Multiplexing & Diagnostics)

    Perfectly suited for next-generation molecular diagnostics, multiplex detection platforms, and advanced gene-editing applications.

 

Storage

Store at -20°C. Upon receipt, aliquoting is highly recommended to avoid repeated freeze-thaw cycles.

 

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Only for research and not intended for treatment of humans or animals
 
 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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