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Cat. No.: PFAGO-100 (for 100 μl)

Cat. No.: PFAGO-500 (for 500 μl)

Description

PfAgo, derived from recombinant expression in Escherichia coli, is a programmable DNA endonuclease. Under the guidance of a short 16-18 nucleotides of 5'-phosphorylated single-stranded DNA (gDNA), the corresponding specific sequence on the substrate is digested, and a break is introduced in the phosphodiester main chain of the complementary substrate sequence. 

The advantage of PfAgo is that the guiding design and target sequence selection are not limited by PAM, and it has high activity on single-stranded DNA and most double-stranded DNA substrates. In the presence of Mg²⁺ or Mn²⁺ metal ions, the enzyme obtains RNase H like activity and cuts the substrate at the base complementary to the 10^th and 11^th bases of the DNA guide chain.

Components

• PfAgo (1 µM): 100 μl
• 10 × Reaction buffer: 50 μl
• MnCl₂ (25 mM): 20 μl

Features

• High sensitivity
• Good specificity
• High flux

Applications

• Target nucleic acid molecular detection
• Gene editing
• Directed digestion

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.