Go Back
PfAgo (Lyophilized)

PfAgo (Lyophilized)

$1,320.00 - $5,280.00
PfAgo is an Argonaute protein derived from the hyperthermophilic archaeon Pyrococcus furiosus, exhibiting programmable endonuclease activity. Guided by a 5'-phosphorylated single-stranded DNA (gDNA), it specifically recognizes and cleaves complementary target DNA sequences. The guide DNA (gDNA) typically consists of a 16–18 nt, 5'-phosphorylated single-stranded oligonucleotide. The cleavage site is precisely located between the 10th and 11th nucleotides from the 5' end of the complementary guide sequence. This version is supplied in lyophilized form. It can be shipped at ambient temperature to reduce transportation costs.
Select
Quantity
Add to cart
Add to cart
More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: PFAGOL-96 (for 96T)

Cat. No.: PFAGOL-960 (for 96T*10)

 

Description

PfAgo is an Argonaute protein derived from the hyperthermophilic archaeon Pyrococcus furiosus, exhibiting programmable endonuclease activity. Guided by a 5'-phosphorylated single-stranded DNA (gDNA), it specifically recognizes and cleaves complementary target DNA sequences. The guide DNA (gDNA) typically consists of a 16–18 nt, 5'-phosphorylated single-stranded oligonucleotide. The cleavage site is precisely located between the 10th and 11th nucleotides from the 5' end of the complementary guide sequence. 

This version is supplied in lyophilized form. It can be shipped at ambient temperature to reduce transportation costs.

 

Components

  • PfAgo Lyophilized Microbead 
  • PfAgo Reaction Buffer
  • Positive Control

 

Key Features of PfAgo

  • Thermophilic Activity (Optimal Temperature ~95°C)

    Exhibits exceptional thermostability with peak catalytic activity at around 95°C, making it ideal for high-temperature molecular workflows.

  • PAM-Independent Cleavage (Distinct from CRISPR Systems)

    Requires no Protospacer Adjacent Motif (PAM) sequences, granting total flexibility in target site selection across the entire genome.

  • DNA-Guided Precision Targeting

    Utilizes cost-effective, highly stable 5'-phosphorylated short single-stranded DNA (gDNA) guides for flawless target recognition.

  • Dual-Substrate Cleavage Capability (dsDNA & ssDNA)

    Capable of cleaving both double-stranded and single-stranded DNA targets with high endonuclease efficiency.

  • Versatile Application Scenarios (Multiplexing & Diagnostics)

    Perfectly suited for next-generation molecular diagnostics, multiplex detection platforms, and advanced gene-editing applications.

 

Storage

Store at -20°C.

 

 

SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.

 

Related: 

 

 

 

Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More