Our CRISPR-dCas9 Nuclese is from Streptococcus pyogenes. The PAM sequence of dCas9 is 5-NGG, which is highly abundant in the genome. dCas9 protein is a ribozyme deficient Cas9 protein, which does not have sgRNA guided DNA strand cleavage activity, but retains target DNA binding activity.
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Cat. No.: dCas-500
Compared with the mRNA or plasmid systems:
Transcription and translation processes are not required