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Cat. No.: MPH1-500 (for 500pmol)

Cat. No.: MPH1-2500 (for 2500pmol)

Description

MS2‑p65‑HSF1 is a key auxiliary protein component of the Synergistic Activation Mediator (SAM) CRISPRa system, representing a major innovation in second‑generation CRISPR activation technologies. This fusion protein consists of three functional modules: the MS2 bacteriophage coat protein, the NF‑κB p65 transcriptional activation domain, and the heat shock factor 1 (HSF1) activation domain. The MS2 domain specifically recognizes and binds MS2 aptamer sequences embedded within RNA stem‑loop structures, while p65 and HSF1 provide strong transcriptional activation capacity.

In the SAM system, the conventional sgRNA is engineered to include MS2 aptamer sequences within its stem‑loop region. When dCas9‑VP64 is recruited to the promoter of a target gene, these modified sgRNAs simultaneously expose MS2 binding sites, enabling efficient recruitment of the MS2‑p65‑HSF1 fusion protein. The cooperative action of VP64, p65, and HSF1 results in a synergistic enhancement of transcriptional activation, dramatically improving gene‑upregulation efficiency compared with first‑generation CRISPRa systems.