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Cat. No.: LtCas12a-100 (for 100pmol)

Cat. No.: LtCas12a-500 (for 500pmol)

Cat. No.: LtCas12a-2500 (for 2500pmol)

Description

LtCas12a is a thermophilic type V‑A CRISPR nuclease derived from Lachnospiraceae thermophila, representing a unique member of the Cas12a family with distinct thermostability advantages. The protein fully retains the hallmark biochemical features of Cas12a nucleases: recognition of a T‑rich PAM sequence (5'-TTTV-3'), sequential cleavage of double‑stranded DNA via a single RuvC nuclease domain, and generation of 5–7 nt 5' overhangs. LtCas12a also possesses intrinsic crRNA processing capability and exhibits trans‑cleavage activity upon target recognition.

The defining characteristic of LtCas12a is its thermophilic adaptation—the enzyme maintains robust nuclease activity and structural stability under elevated temperatures. This property expands its utility in specialized applications where conventional mesophilic Cas12a variants are less effective, such as high‑temperature genome editing, thermostable diagnostic platforms, and environments requiring enhanced protein stability.