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FnCas12a Nuclease (Lyophilized)

FnCas12a Nuclease (Lyophilized)

$2,250.00 - $8,750.00
FnCas12a (also known as Cpf1) nuclease is derived from the strain Francisella tularensis. FnCas12a is a crRNA-guided DNA endonuclease. In the presence of a PAM (TTN) sequence on the target double-stranded DNA, it specifically cleaves the target double-stranded DNA, inducing double-strand breaks with sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by FnCas12a is PAM-independent. Once FnCas12a assembles into a ternary complex with crRNA and target DNA, it triggers collateral cleavage of non-specific single-stranded DNA (ssDNA), degrading any ssDNA sequences present in the reaction system. For this reason, FnCas12a can be applied for rapid detection of target nucleic acids.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: FC12Lyo-100 (for 100μg)

Cat. No.: FC12Lyo-500 (for 500μg)

 

Description

FnCas12a (also known as Cpf1) nuclease is derived from the strain Francisella tularensis. FnCas12a is a crRNA-guided DNA endonuclease. In the presence of a PAM (TTN) sequence on the target double-stranded DNA, it specifically cleaves the target double-stranded DNA, inducing double-strand breaks with sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by FnCas12a is PAM-independent. Once FnCas12a assembles into a ternary complex with crRNA and target DNA, it triggers collateral cleavage of non-specific single-stranded DNA (ssDNA), degrading any ssDNA sequences present in the reaction system. For this reason, FnCas12a can be applied for rapid detection of target nucleic acids.

This product is glycerol-free lyophilized FnCas12a nuclease, which allows transportation and storage at ambient temperature. It is available in multiple formats including lyophilized powder and lyophilized beads to accommodate diverse application scenarios.

 

Components

  • FnCas12a Nuclease (Lyophilized) 
  • Cas Reconstitution Solution
  • 10× Buffer for Cas12a

 

Reconstitution Recommendation 

Dissolve 100 μg of lyophilized FnCas12a in 130 μL of reconstitution solution to obtain a final enzyme concentration of 5 μM (5 pmol/μL).

 

Storage

  • FnCas12a Nuclease (Lyophilized) + Cas Reconstitution Solution: Stable at room temperature for storage and transport. After reconstitution, store at –20 °C and avoid repeated freeze–thaw cycles.
  • 10× Buffer for FnCas12a​: Store at –20 °C; transport at 4 °C.

 

 

SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.

 

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Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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