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Cat. No.: dPC13A-500 (for 500pmol)

Cat. No.: dPC13A-2500 (for 2500pmol)

Description

dPspCas13b‑ADAT2 is an RNA‑editing tool based on CRISPR technology and serves as a core component of the REPAIR (RNA Editing for Programmable A‑to‑I Replacement) system. This fusion protein combines catalytically inactivated Prevotella sp. P5‑125 Cas13b (dPspCas13b) with the adenosine deaminase ADAT2 domain, enabling precise base editing at the RNA level and offering a novel strategy for correcting pathogenic RNA mutations.

Cas13b is a type VI‑B CRISPR effector naturally specialized for RNA targeting. By introducing key mutations that inactivate its HEPN nuclease domains, dPspCas13b completely loses RNA‑cleavage activity while retaining its ability to bind target RNA sequences with high specificity under the guidance of CRISPR RNA (crRNA). This makes it an accurate and programmable RNA‑targeting scaffold.

ADAT2 is an adenosine deaminase involved in tRNA modification and catalyzes the hydrolytic deamination of adenosine (A) to inosine (I). Because inosine is interpreted as guanosine (G) during translation, A‑to‑I editing effectively results in a functional A‑to‑G substitution.

When a crRNA directs dPspCas13b‑ADAT2 to a target transcript, the ADAT2 domain performs localized deamination of specific adenosines, enabling precise recoding of RNA sequences without altering the underlying genome.