All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: dC9GVP-500 (for 500pmol)

Cat. No.: dC9GVP-2500 (for 2500pmol)

Description

dCas9‑GFP‑VP64 is a multifunctional chimeric protein that integrates genomic targeting, real‑time visualization, and transcriptional activation. It consists of catalytically inactive Cas9 (dCas9), green fluorescent protein (GFP), and the VP64 transcriptional activation domain. The dCas9 component carries the D10A and N863A mutations, which completely abolish nuclease activity while preserving precise DNA‑binding capability directed by a single‑guide RNA (sgRNA).

GFP serves as a fluorescent reporter, providing a bright and stable signal that enables real‑time tracking of the protein’s subcellular localization, dynamic distribution, and target‑binding behavior. The VP64 domain—composed of four tandem copies of the herpes simplex virus VP16 activation motif—functions as a potent recruiter of transcriptional machinery.

When guided by a sequence‑specific sgRNA to a promoter region, dCas9‑GFP‑VP64 brings the VP64 activation module to the target locus. VP64 then recruits components of the transcription pre‑initiation complex, histone acetyltransferases, and other co‑activators, promoting RNA polymerase II assembly and transcription initiation. This results in robust upregulation of the target gene.