All products have special prices for bulk purchase, please contact us for more details if required.

The lyophilized Cas13a is available and can be shipped at room temperature. Please visit Cas13a Nuclease (Lyophilized) for more details.

LtrCas13a and LbuCas13a are also available. Please contact us for more details if required.

Cat. No.: LwaCas13a-1k (for 1000pmol)

Cat. No.: LwaCas13a-5k (for 5000pmol)

Description

LwaCas13a (also known as C2c2) is a crRNA-guided RNA endonuclease dependent on crRNA alone, originating from the Leptotrichia wadei strain. It can specifically recognize and cleave target single-stranded RNA, with relatively lenient requirements for the PFS sequence. Additionally, Cas13a exhibits trans-cleavage activity (i.e., collateral cleavage activity). Upon formation of a ternary complex comprising LwaCas13a protein, crRNA, and target RNA, the enzyme is activated to cleave non-specific sequences of single-stranded RNA in the system, breaking down any sequence of single-stranded RNA. This activity has also been utilized in the development of rapid detection kits for target nucleic acids.

Applications

• Real-time Fluorescent Detection 
• Colloidal gold chromatography
• Gene editing
• Molecular diagnosis

Storage