All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: PFAGOE-200 (for 200pmol)

Cat. No.: PFAGOE-2k (for 2,000pmol )

Description

PfAgo is an Argonaute protein derived from the hyperthermophilic archaeon Pyrococcus furiosus, exhibiting programmable endonuclease activity. Guided by a 5'-phosphorylated single-stranded DNA (gDNA), it specifically recognizes and cleaves complementary target DNA sequences. The guide DNA (gDNA) typically consists of a 16–18 nt, 5'-phosphorylated single-stranded oligonucleotide. The cleavage site is precisely located between the 10th and 11th nucleotides from the 5' end of the complementary guide sequence.

Key Features of PfAgo

• Thermophilic Activity (Optimal Temperature ~95°C)

  Exhibits exceptional thermostability with peak catalytic activity at around 95°C, making it ideal for high-temperature molecular workflows.

• PAM-Independent Cleavage (Distinct from CRISPR Systems)

  Requires no Protospacer Adjacent Motif (PAM) sequences, granting total flexibility in target site selection across the entire genome.

• DNA-Guided Precision Targeting

  Utilizes cost-effective, highly stable 5'-phosphorylated short single-stranded DNA (gDNA) guides for flawless target recognition.

• Dual-Substrate Cleavage Capability (dsDNA & ssDNA)

  Capable of cleaving both double-stranded and single-stranded DNA targets with high endonuclease efficiency.

• Versatile Application Scenarios (Multiplexing & Diagnostics)

  Perfectly suited for next-generation molecular diagnostics, multiplex detection platforms, and advanced gene-editing applications.

Storage

Store at -20°C. Upon receipt, aliquoting is highly recommended to avoid repeated freeze-thaw cycles.

Related: 

• Synthetic sgRNAs
• Cas12a Nuclease (Lyophilized)
• Cas12b Nuclease (Lyophilized)
• Cas13a Nuclease (Lyophilized)
• Cas14a1 Nuclease (Lyophilized)
• SpCas9 Nuclease (Lyophilized)
• SuCas12a2 Nuclease (Lyophilized)
• Cas9 Nuclease (with NLS)
• Cas9 HF-NLS (SpCas9 HF-NLS)
• CRISPR-dCas9 Nuclease
• dCas9 NLS
• spCas9
• SpdCas9
• spCas9-NG
• SpRYCas9
• Cas9 Nickase (H840A) NLS
• Cas9 Nickase (D10A) NLS
• TsCas12a
• OsCas12a
• HkCas12a
• BoCas12a
• AsCas12a
• FnCas12a
• LbCas12a (Cpf1) Nuclease
• Enhanced LbaCas12a (Cpf1)
• Lb5Cas12a (Cpf1) Nuclease
• SuCas12a2 Nuclease
• AaCas12b
• AacCas12b
• AapCas12b
• BrCas12b
• LwCas13a
• LbuCas13a
• LtrCas13a (C2c2) Nuclease
• LwaCas13a (C2c2) Nuclease
• EiCsm6
• PfAgo
• TtAgo
• T7 Endonuclease I