All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DCas9NL-100 (for 100pmol)

Cat. No.: DCas9NL-500 (for 500pmol)

Description

dCas9 NLS is a nuclease with a Nuclear Localization Signal (NLS) that specifically binds double-stranded DNA under the guidance of gRNA without cutting it. dCas9 NLS is a D10A/H840A double mutant created by amino acid mutations in the RuvC and HNH domains of the Cas9 nuclease. Like the wild-type Cas9 nuclease, dCas9 NLS targets the DNA sequence with the help of gRNA. However, due to mutations in the RuvC and HNH domains, dCas9 NLS has gRNA-guided DNA binding activity without endonuclease activity. Studies have shown that when dCas9 NLS is combined with proteins of different functions, it can effectively inhibit or promote gene expression. At the same time, dCas9 NLS provides a platform for these proteins to bind to specific genomic sites.

dCas9 NLS contains Nuclear Localization Signals (NLS) of SV40 T antigen at both the N-terminus and C-terminus of the protein. This allows the dCas9 NLS and gRNA complex to quickly enter the nucleus from the cytoplasm after transfection. dCas9 NLS can enter cells via microinjection, electroporation, and liposome-mediated methods, and this DNA-free system eliminates the risk of foreign DNA integration into the cell genome.

Source

Obtained through recombinant expression and purification in E. coli, with the gene source from Streptococcus pyogenes. 

Applications

Gene expression regulation; genomic site imaging; gene enrichment, etc. 

Purity

Purity ≥95% after SDS-PAGE electrophoresis; free from DNA exonucleases, DNA endonucleases, RNAase, and phospholipase.

Storage