In a 50 μL reaction system, at 37℃ for 1 h, the amount of enzyme required to convert more than 90% of the pUC(AT)* of a 1-ug supercoiled cross-shaped structure into more than 90% of the linear structure is defined as an active unit.
- Detection of mutant caused by gene mutation, SNP, TALEN or CRISPR/Cas9
- Recognition of mismatched DNA
- Decomposition of four-way junction DNA or Branched DNA
- Detection or cutting of heterodimer DNA
- Shot-gun cloning by random cutting of linear DNA
1X T7 Endonuclease I Buffer
50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.9 at 25℃).
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5.
Stored at -20℃ for 2 years. Avoid repeated freezing and thawing.
- T7 endonuclease I is an enzyme with substrate structure selectivity. It acts on different DNA substrates with different activities. To cut a specific substrate, the amount of enzyme and reaction time must be carefully controlled.
- When the reaction temperature exceeds 42℃, the non-specific nuclease activity will be increased.