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Cat. No.: GeCas12a2-100 (for 100pmol)

Cat. No.: GeCas12a2-1k (for 1000pmol)

Description

GeCas12a2 nuclease was screened from environmental metagenomes rather than from a single isolated and purified known axenic strain. Its amino acid sequence shares 89% identity with SuCas12a2. Cas12a2 is a crRNA-guided endonuclease that targets RNA sequences, and the direct repeat (DR) sequence of its crRNA is largely consistent with that of Cas12a. Under the guidance of crRNA, GeCas12a2 assembles into a ternary complex together with crRNA and target ssRNA, enabling specific cleavage of the target ssRNA. Meanwhile, GeCas12a2 is activated to exert trans-cleavage activity toward unpaired ssDNA, ssRNA and dsDNA, indiscriminately degrading any ssDNA, ssRNA and dsDNA sequences present in the system. Unlike other Cas nucleases, which only perform trans-cleavage on ssDNA (e.g., Cas12a, Cas12b) or ssRNA (e.g., Cas13a) upon target activation, GeCas12a2 exhibits substantially stronger non-specific cleavage activity.

Storage