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Cat. No.: 3MSLE-500 (for 500Tx10µl)

Cat. No.: 3MSLE-5k (for 5000Tx10µl)

Description

3M Spherical Lyophilization Excipient is a 3× concentration lyophilization excipient, containing matrix-forming agents and enzyme stabilizers, specifically designed for the spherical freeze-drying of designated product lines. It is not intended for use with unspecified experimental applications.

Compatible Reagents

• PrimeIAmp™ HotStart Bst P DNA/RNA LHNB LyoMix (70ºC reaction)​
• PrimeIAmp™ HotStart Bst P DNA/RNA Basic & SYBR Green LyoMix (70ºC reaction)
• PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)
• PrimeIAmp™ HotStart Bst DNA/RNA Basic & SYBR Green LyoMix (65ºC reaction)

Note: This list will be updated regularly. Please refer to the relevant website for the latest information.

Reconstitution

Due to the high concentration of solid content, the product may be difficult to dissolve. Thoroughly reconstitute by incubating in a 37°C water bath before use.

Storage

Store below –20°C for up to 36 months. The product maintains its performance after up to 10 freeze–thaw cycles. When stored at 2–8°C, it remains stable for 4 weeks.

Related:

• 5× CM HS-G3Taq SYBR Green qPCR Mix
• 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
• CM HS-G3Taq Probe qPCR Lyophilized Bead
• 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
• CM HS-G3mTTx qRT-PCR Lyophilized Bead
• 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
• DualHS G3mTTx qRT-PCR Lyophilized Bead
• 3M Spherical Lyophilization Excipient
• 1.67x Primer Bead Lyophilization Excipient

The G3mTTx DNA/RNA qPCR Mix contains SBS Genetech’s engineered Extreme ThermoStable Reverse Transcriptase (ET RTase). This enzyme withstands 95 °C and efficiently reverse transcribes RNA up to 500 nt in length. Unlike conventional reverse transcriptases restricted to 37–50 °C, ET RTase enables cDNA synthesis at virtually any temperature, determined only by primer annealing conditions.

In addition, the system allows one-tube processing of crude samples—heat lysis, nucleic acid release, reverse transcription, and PCR amplification—streamlining workflows and redefining the format of molecular diagnostic reagents and instrumentation.

ET RTase is derived from a thermostable DNA polymerase and re-engineered using electronic scaffold reconstruction technology. Its ligand-binding core was modified to eliminate Mn²⁺ dependence, achieving strong reverse transcription activity with Mg²⁺ alone. This innovation removes the inhibitory effects of Mn²⁺ on PCR.

SBS Genetech provides two versions of the G3mTTx Mix:

• CM Version – More flexible and user-friendly.

• DualHS Version – Requires slightly stricter handling but delivers more rigorous amplification control.

Both formulations are glycerol-free. When combined with SBS Genetech’s proprietary 3M Spherical Lyophilization Excipient, they can be readily prepared into lyophilized beads containing amplification primers. This integrated design simplifies research, development, and production workflows while maintaining flexibility and reducing the complexity of reagent development and fixed-format manufacturing.

SBS Genetech also offers customized production of qPCR lyophilized beads, tailored to specific research or diagnostic needs.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory