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Cat. No.: CMHPG3-250 (for 200Tx20µl)

Cat. No.: CMHPG3-1k (for 1000Tx20µl)

Description

5× CM HS-G3Taq Probe Mix (Lyo-Ready) is a specialized reagent for Real-Time PCR using the TaqMan probe-based method. It contains chemically modified HotStart 3G-Taq DNA Polymerase (fully heat-activated), reaction buffer, dNTPs, and stabilizers, formulated as a 5× single-component master mix that does not include ROX dye. The optimized buffer system enables its use in multiplex quantitative PCR assays of up to four targets.

The 5× CM HS-G3Taq Probe Mix (Lyo-Ready) is glycerol-free, allowing seamless integration with our proprietary 3M Spherical Lyophilization Excipient for the easy preparation of lyophilized primer-containing PCR beads. This combination design streamlines the workflow for research, development, and manufacturing, while maintaining flexibility and reducing the complexity of reagent formulation and immobilization.

The third-generation G3Taq DNA Polymerase demonstrates exceptional resistance to inhibitors such as ethanol, guanidine salts, and heparin, making it suitable for use with low-purity DNA templates. The CM HS-G3Taq DNA Polymerase, a chemically modified HotStart variant, remains completely inactive below 50°C and requires heating at 95°C for 5 minutes to fully restore enzymatic activity. This ensures effective suppression of nonspecific amplification, significantly improving PCR specificity.

Features

• Amplification Performance: Capable of amplifying DNA fragments up to 4 kb from λDNA and up to 2 kb from human genomic DNA.
• High Specificity: Remains completely inactive below 50°C and requires heating at 95°C for 5 minutes to fully restore enzymatic activity.

Unit Definition 

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage 

Stable for up to 3 years at -20°C.

Performance Comparison of HotStart G3Taq Polymerases

Related: 

• CM HS-G3Taq DNA Polymerase (Glycerol Free)
• 5× CM HS-G3Taq SYBR Green qPCR Mix
• 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
• 5×​ CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
• 3M Spherical Lyophilization Excipient
• AP HS-G3Taq DNA Polymerase (Glycerol Free)
• 5×​ DualHS G3mTTx qPCR Mix (Lyo-Ready)

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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