The CM HS-G3mTTx qRT-PCR Lyophilized Bead contains chemically blocked hot-start G3Taq DNA polymerase, Extreme ThermoStable Reverse Transcriptase (ET RTase), dNTPs, Mg²⁺, and lyophilization excipients. Under Mg²⁺ conditions alone (no Mn²⁺ required), the formulation enables unbiased amplification of both DNA and RNA.

This reagent is a dedicated TaqMan PCR premix (ROX-free) that allows highly sensitive detection of DNA and RNA molecules. Thanks to the exceptional thermostability of ET RTase, which remains fully active at 95 °C, both DNA and RNA viruses can be processed by simple heat treatment to release nucleic acids for subsequent PCR amplification. As a result, crude samples can be directly tested without nucleic acid purification.

• Store sealed and dry at ≤ –20 °C: stable for 60 months.

• Store sealed and dry at room temperature: stable for 12 months.

• As the product is lyophilized and highly hygroscopic, once opened it is recommended to store at ≤ –20 °C, where it remains stable for 24 months.

Note

• Enzyme Activation

  • The 3G Taq enzyme is chemically modified for hot-start function.

  • Enzyme activity is restored only after heating at 95 °C for 5 min.

  • The heating time must not be shortened.

• Physical Specification

  • Lyophilized bead diameter: ~2.6 mm.

Related:

• 5× CM HS-G3Taq SYBR Green qPCR Mix
• 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
• CM HS-G3Taq Probe qPCR Lyophilized Bead
• 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
• CM HS-G3mTTx qRT-PCR Lyophilized Bead
• 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
• DualHS G3mTTx qRT-PCR Lyophilized Bead
• 3M Spherical Lyophilization Excipient
• 1.67x Primer Bead Lyophilization Excipient

The G3mTTx DNA/RNA qPCR Mix contains SBS Genetech’s engineered Extreme ThermoStable Reverse Transcriptase (ET RTase). This enzyme withstands 95 °C and efficiently reverse transcribes RNA up to 500 nt in length. Unlike conventional reverse transcriptases restricted to 37–50 °C, ET RTase enables cDNA synthesis at virtually any temperature, determined only by primer annealing conditions.

In addition, the system allows one-tube processing of crude samples—heat lysis, nucleic acid release, reverse transcription, and PCR amplification—streamlining workflows and redefining the format of molecular diagnostic reagents and instrumentation.

ET RTase is derived from a thermostable DNA polymerase and re-engineered using electronic scaffold reconstruction technology. Its ligand-binding core was modified to eliminate Mn²⁺ dependence, achieving strong reverse transcription activity with Mg²⁺ alone. This innovation removes the inhibitory effects of Mn²⁺ on PCR.

SBS Genetech provides two versions of the G3mTTx Mix:

• CM Version – More flexible and user-friendly.

• DualHS Version – Requires slightly stricter handling but delivers more rigorous amplification control.

Both formulations are glycerol-free. When combined with SBS Genetech’s proprietary 3M Spherical Lyophilization Excipient, they can be readily prepared into lyophilized beads containing amplification primers. This integrated design simplifies research, development, and production workflows while maintaining flexibility and reducing the complexity of reagent development and fixed-format manufacturing.

SBS Genetech also offers customized production of qPCR lyophilized beads, tailored to specific research or diagnostic needs.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory