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CM HS-G3Taq Probe qPCR Lyophilized Bead

CM HS-G3Taq Probe qPCR Lyophilized Bead

$400.00 - $1,200.00
The CM HS-G3Taq Probe qPCR Lyophilized Bead is a specialized reagent for Real-Time PCR using the TaqMan probe method. Each bead contains chemically modified hot-start G3Taq DNA polymerase (100% hot-start), optimized reaction buffer, dNTPs, Mg²⁺, and lyophilization excipients (ROX-free). The optimized formulation enables reliable performance in singleplex to four-plex probe-based quantitative PCR assays.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: CMLB-96 (for 96T)

Cat. No.: CMLB-480 (for 480T)

 

Description

The CM HS-G3Taq Probe qPCR Lyophilized Bead is a specialized reagent for Real-Time PCR using the TaqMan probe method. Each bead contains chemically modified hot-start G3Taq DNA polymerase (100% hot-start), optimized reaction buffer, dNTPs, Mg²⁺, and lyophilization excipients (ROX-free). The optimized formulation enables reliable performance in singleplex to four-plex probe-based quantitative PCR assays.

 

Key Features

  1. Third-Generation DNA Polymerase

    • G3Taq DNA polymerase represents the third generation of Taq enzymes.

    • Exhibits exceptional tolerance to impurities such as ethanol, guanidine salts, and heparin.

    • Ensures robust results even with low-purity DNA templates.

  2. Chemically Modified Hot-Start System

    • RAPA3G DNA polymerase is chemically modified to achieve 100% hot-start control.

    • Completely inactive below 50 °C.

    • Enzyme activity is fully restored only after heating at 95 °C for 5 min.

    • This mechanism effectively suppresses non-specific amplification and significantly enhances PCR specificity.

  3. Optimized Multiplex Capability

    • Reaction system supports 1–4 probe multiplex qPCR with high sensitivity and specificity.

 

Storage

  • Store sealed and dry at ≤ –20 °C: stable for 60 months.

  • Store sealed and dry at room temperature: stable for 12 months.

  • As the product is lyophilized and highly hygroscopic, once opened it is recommended to store at ≤ –20 °C, where it remains stable for 24 months.

 

Note

  • Enzyme Activation

    • The 3G Taq enzyme is chemically modified for hot-start function.

    • Enzyme activity is restored only after heating at 95 °C for 5 min.

    • The heating time must not be shortened.

  • Physical Specification

    • Lyophilized bead diameter: ~2.6 mm.

 

Related:

 

The G3mTTx DNA/RNA qPCR Mix contains SBS Genetech’s engineered Extreme ThermoStable Reverse Transcriptase (ET RTase). This enzyme withstands 95 °C and efficiently reverse transcribes RNA up to 500 nt in length. Unlike conventional reverse transcriptases restricted to 37–50 °C, ET RTase enables cDNA synthesis at virtually any temperature, determined only by primer annealing conditions.

In addition, the system allows one-tube processing of crude samples—heat lysis, nucleic acid release, reverse transcription, and PCR amplification—streamlining workflows and redefining the format of molecular diagnostic reagents and instrumentation.

ET RTase is derived from a thermostable DNA polymerase and re-engineered using electronic scaffold reconstruction technology. Its ligand-binding core was modified to eliminate Mn²⁺ dependence, achieving strong reverse transcription activity with Mg²⁺ alone. This innovation removes the inhibitory effects of Mn²⁺ on PCR.

SBS Genetech provides two versions of the G3mTTx Mix:

  • CM Version – More flexible and user-friendly.

  • DualHS Version – Requires slightly stricter handling but delivers more rigorous amplification control.

Both formulations are glycerol-free. When combined with SBS Genetech’s proprietary 3M Spherical Lyophilization Excipient, they can be readily prepared into lyophilized beads containing amplification primers. This integrated design simplifies research, development, and production workflows while maintaining flexibility and reducing the complexity of reagent development and fixed-format manufacturing.

SBS Genetech also offers customized production of qPCR lyophilized beads, tailored to specific research or diagnostic needs.

 

ProductCM HS-G3 Probe MixCM G3mTTx MixDualHS G3mTTx Mix
  • Template Type
  • DNA
  • DNA/RNA (universal)
  • DNA/RNA (universal)
  • Taq Polymerase Hot-Start
  • Chemically blocked
  • Chemically blocked
  • Dual-blocked (aptamer + Mg²⁺)
  • Reverse Transcriptase
  • None
  • ET RTase (stable at 95 °C)
  • ET RTase (stable at 95 °C)
  • PCR Program (Purified Nucleic Acids)
  • 95 °C 5 min (hot-start)
  • 40× (95 °C 10 s, 60 °C 30 s)
  • 50–75 °C 10 min (reverse transcription)
  • 95 °C 5 min (hot-start)
  • 40× (95 °C 10 s, 60 °C 30 s)
  • 95 °C 2 min (Mg²⁺ activation)
  • 50–75 °C 10 min (reverse transcription)
  • 40× (95 °C 10 s, 60 °C 30 s)
  • PCR Program (Unpurified Nucleic Acids)
  • 95 °C 5 min (nucleic acid release + hot-start)
  • 40× (95 °C 10 s, 60 °C 30 s)
  • 95 °C 5 min (nucleic acid release + hot-start)
  • 50–75 °C 10 min (reverse transcription)<
  • 40× (95 °C 10 s, 60 °C 30 s)
  • 95 °C 2 min (nucleic acid release + Mg²⁺ activation)
  • 50–75 °C 10 min (reverse transcription)
  • 40× (95 °C 10 s, 60 °C 30 s)

 

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

 

 

Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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