1.67x Primer Bead Lyophilization Excipient
$150.00 - $1,000.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: PBLE-500 (for 500Tx5µl)
Cat. No.: PBLE-5000 (for 5,000Tx5µl)
Description
The 1.67x Primer Bead Lyophilization Excipient is a 1.67× concentrated lyophilization excipient containing bead-forming agents and enzyme stabilizers. It is specifically designed for primer bead lyophilization, with a bead volume of 5 µl (approx. 2 mm diameter).
Package Sizes
Product | 500T × 5 µl | 5000T × 5 µl |
|---|---|---|
|
|
|
Storage & Stability
Store at –20 °C (stable for 36 months).
Tolerates up to 10 freeze–thaw cycles without performance loss.
Stable for 4 weeks at 2–8 °C.
High solid content; dissolve thoroughly at 37 °C before use.
Recommended Use
Lyophilization Mix Preparation
1.67xY05 Primer Bead: 3 µl
Primer Mix: 2 µl
Total: 5 µl
Bead Formation
Dispense 5 µl droplets using a bead dispenser.
Complete dispensing within 1 h.
Beads can be directly lyophilized or pre-frozen at –50 °C for 5 min before lyophilization.
Lyophilization Program (for reference only; may vary by equipment)
Pre-freeze at –50 °C for 5–10 min
–50 °C for 2 h (vacuum stage)
Ramp from –50 °C to 30 °C at 5 °C/h
Hold at 30 °C for 4 h
Maintain at 30 °C until completion
Related:
- 5× CM HS-G3Taq SYBR Green qPCR Mix
- 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
- CM HS-G3Taq Probe qPCR Lyophilized Bead
- 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
- CM HS-G3mTTx qRT-PCR Lyophilized Bead
- 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
- DualHS G3mTTx qRT-PCR Lyophilized Bead
- 3M Spherical Lyophilization Excipient
- 1.67x Primer Bead Lyophilization Excipient
The G3mTTx DNA/RNA qPCR Mix contains SBS Genetech’s engineered Extreme ThermoStable Reverse Transcriptase (ET RTase). This enzyme withstands 95 °C and efficiently reverse transcribes RNA up to 500 nt in length. Unlike conventional reverse transcriptases restricted to 37–50 °C, ET RTase enables cDNA synthesis at virtually any temperature, determined only by primer annealing conditions.
In addition, the system allows one-tube processing of crude samples—heat lysis, nucleic acid release, reverse transcription, and PCR amplification—streamlining workflows and redefining the format of molecular diagnostic reagents and instrumentation.
ET RTase is derived from a thermostable DNA polymerase and re-engineered using electronic scaffold reconstruction technology. Its ligand-binding core was modified to eliminate Mn²⁺ dependence, achieving strong reverse transcription activity with Mg²⁺ alone. This innovation removes the inhibitory effects of Mn²⁺ on PCR.
SBS Genetech provides two versions of the G3mTTx Mix:
CM Version – More flexible and user-friendly.
DualHS Version – Requires slightly stricter handling but delivers more rigorous amplification control.
Both formulations are glycerol-free. When combined with SBS Genetech’s proprietary 3M Spherical Lyophilization Excipient, they can be readily prepared into lyophilized beads containing amplification primers. This integrated design simplifies research, development, and production workflows while maintaining flexibility and reducing the complexity of reagent development and fixed-format manufacturing.
SBS Genetech also offers customized production of qPCR lyophilized beads, tailored to specific research or diagnostic needs.
| Product | CM HS-G3 Probe Mix | CM G3mTTx Mix | DualHS G3mTTx Mix |
|---|---|---|---|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
