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Cat. No.: CMHSG3-250 (for 200Tx20µl)

Cat. No.: CMHSG3-1k (for 1000Tx20µl)

Description

5× CM HS-G3Taq SYBR Green qPCR Mix is a specialized reagent for Real-Time PCR using the SYBR Green intercalating dye method. The SYBR Green qPCR Mix is a 5× single-component master mix that includes chemically modified HotStart G3Taq DNA Polymerase (fully heat-activated), reaction buffer, dNTPs, SYBR Green dye, and other necessary components pre-mixed for convenience.

The G3Taq DNA Polymerase, a third-generation polymerase, offers exceptional tolerance to common inhibitors such as ethanol, guanidine salts, and heparin. This allows the mix to deliver robust results even when working with impure DNA templates. The CM HS-G3Taq DNA Polymerase is a chemically modified HotStart enzyme that remains completely inactive below 50°C and only regains full activity after heating at 95°C for 5 minutes. This feature effectively suppresses nonspecific amplification, dramatically improving PCR specificity.

The kit includes a proprietary buffer system optimized to deliver strong amplification performance across a broad range of conditions, with enhanced sensitivity and signal intensity.

Features

• Amplification Performance: Capable of amplifying DNA fragments up to 4 kb from λDNA and up to 2 kb from human genomic DNA.
• High Specificity: Remains completely inactive below 50°C and requires heating at 95°C for 5 minutes to fully restore enzymatic activity.

Unit Definition 

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage 

Stable for up to 3 years at -20°C.

Performance Comparison of HotStart G3Taq Polymerases

Related:

• 5× CM HS-G3Taq SYBR Green qPCR Mix
• 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
• CM HS-G3Taq Probe qPCR Lyophilized Bead
• 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
• CM HS-G3mTTx qRT-PCR Lyophilized Bead
• 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
• DualHS G3mTTx qRT-PCR Lyophilized Bead
• 3M Spherical Lyophilization Excipient
• 1.67x Primer Bead Lyophilization Excipient

The G3mTTx DNA/RNA qPCR Mix contains SBS Genetech’s engineered Extreme ThermoStable Reverse Transcriptase (ET RTase). This enzyme withstands 95 °C and efficiently reverse transcribes RNA up to 500 nt in length. Unlike conventional reverse transcriptases restricted to 37–50 °C, ET RTase enables cDNA synthesis at virtually any temperature, determined only by primer annealing conditions.

In addition, the system allows one-tube processing of crude samples—heat lysis, nucleic acid release, reverse transcription, and PCR amplification—streamlining workflows and redefining the format of molecular diagnostic reagents and instrumentation.

ET RTase is derived from a thermostable DNA polymerase and re-engineered using electronic scaffold reconstruction technology. Its ligand-binding core was modified to eliminate Mn²⁺ dependence, achieving strong reverse transcription activity with Mg²⁺ alone. This innovation removes the inhibitory effects of Mn²⁺ on PCR.

SBS Genetech provides two versions of the G3mTTx Mix:

• CM Version – More flexible and user-friendly.

• DualHS Version – Requires slightly stricter handling but delivers more rigorous amplification control.

Both formulations are glycerol-free. When combined with SBS Genetech’s proprietary 3M Spherical Lyophilization Excipient, they can be readily prepared into lyophilized beads containing amplification primers. This integrated design simplifies research, development, and production workflows while maintaining flexibility and reducing the complexity of reagent development and fixed-format manufacturing.

SBS Genetech also offers customized production of qPCR lyophilized beads, tailored to specific research or diagnostic needs.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory