5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
$300.00 - $1,200.00
$1,500.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: CHG3MT-100 (for 100Tx20µl)
Cat. No.: CHG3MT-500 (for 500Tx20µl)
Description
5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready) is a specialized 5× master mix designed for TaqMan probe-based Real-Time PCR, containing chemically blocked HotStart CM HS-G3Taq DNA Polymerase, extremely thermostable reverse transcriptase (ET RTase), dNTPs, Mg²⁺, and stabilizers. The mix enables unbiased amplification of both DNA and RNA targets under magnesium-dependent conditions and supports high-sensitivity detection of nucleic acids from either source.
Thanks to the exceptional heat resistance of ET RTase, capable of withstanding 95°C, nucleic acids can be liberated from DNA or RNA viruses through thermal treatment—allowing direct detection from crude samples without nucleic acid purification.
Features
- HotStart Mechanism: The chemically modified CM HS-G3Taq DNA Polymerase remains fully inactive below 50°C and regains activity only after heating at 95°C for 5 minutes.
- Thermostable Reverse Transcription: ET RTase retains full activity even after 15 minutes at 95°C. Its active site has been engineered to shift metal ion dependence from Mn²⁺ to Mg²⁺, enabling synchronous RT-PCR in a single system.
- Optimized Probe Cleavage: Modification of the TTx polymerase active site reduces its 5'→3' exonuclease activity; the 3G Taq enzyme compensates by providing robust Flap 5'→3' exonuclease activity for effective TaqMan probe cleavage.
- Target Range: When using RNA as the template, the mix supports RT-PCR of fragments up to 500 bp, with optimal amplification efficiency in the range of 70–150 bp.
- Glycerol-Free Lyophilization Compatibility: The 5× CM HS-G3mTTx Mix contains no glycerol, making it compatible with our proprietary 3M Spherical Lyophilization Excipient for easy preparation of lyophilized primer-included PCR beads. This design facilitates streamlined integration of research, development, and manufacturing, while enhancing flexibility and simplifying reagent formulation and immobilization.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
Stable for up to 3 years at -20°C.
Performance Comparison of HotStart G3Taq Polymerases
HotStart Mechanism | CM HS-G3Taq Chemical modification | AP HS-G3Taq Aptamer-based blocking |
Inactivation Efficiency | 100% inactive below 50°C | ~90% inactive below 37°C |
Enzyme Activation | 95°C for 5 minutes | Above 50°C |
Average Extension Rate | 1 kb/min | 2 kb/min |
Amplification Capability | Up to 2 kb (human gDNA) | Up to 8 kb (human gDNA) |
Recommended Applications | Multiplex and short-fragment amplification with high specificity | Long-fragment amplification with high specificity |
Related:
- CM HS-G3Taq DNA Polymerase (Glycerol Free)
- 5× CM HS-G3Taq SYBR Green qPCR Mix
- 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
- 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
- 3M Spherical Lyophilization Excipient
- AP HS-G3Taq DNA Polymerase (Glycerol Free)
- 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
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