PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)
$660.00 - $2,000.00
$2,500.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: IHBLL-100 (for 100T)
Cat. No.: IHBLL-400 (for 400T)
Description
PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix, meticulously developed by SBS Genetech, is tailored for LAMP amplification and supports both DNA and RNA target detection. Based on a fast and highly tolerant Bst DNA/RNA polymerase, it achieves stable, rapid, and highly sensitive amplification. The integration of HotStart technology further reduces the risk of false positives.
Its proprietary lyophilized formulation grants the reagent exceptional versatility—fulfilling routine research needs while also streamlining product development and large-scale lyophilized reagent manufacturing with a unified formulation.
Product Features: HotStart Bst DNA/RNA polymerase is equipped with a heat-activated aptamer that ensures >90% inhibition of enzyme activity below 30 °C and complete activation within 1 minute above 60 °C. This design facilitates room temperature setup and significantly minimizes nonspecific amplification at lower temperatures.
The reagent also contains LHNB (Leuco-HNB), a chromogenic dye that initially forms a deep violet complex with Mg²⁺. As the LAMP reaction proceeds, pyrophosphate (PPi) generated during amplification binds Mg²⁺, leading to the release of free LHNB and a visible color change to pale green.
(LHNB–Mg²⁺ complex) → (PPi–Mg²⁺) + (free LHNB)
Deep Violet Pale Green
Components
- HS-Bst DNA/RNA LyoMixA
- HS-Bst DNA/RNA LyoMixB
- LHNB Dye
Storage
- For long-term storage, keep the product at –20 °C or below (stable for up to 18 months). The reagent can withstand up to 10 freeze–thaw cycles without performance loss, though repeated freeze–thawing should be avoided when possible. For short-term use, storage at 2–8 °C is recommended, where the product remains stable for up to 2 weeks. Due to the high concentration of lyophilization excipients, a small amount of white insoluble material may be observed upon thawing; this will fully dissolve after 3–5 minutes at 30 °C (or by warming in the palm), without affecting product performance.
- SYBR Green dye is photosensitive and prone to degradation. Protect from light during storage and use. The recommended final concentration is 0.75×.
Precautions
- This product contains a high concentration of salts. Appropriate personal protective equipment (PPE) should be worn to avoid contact with skin, eyes, nose, and respiratory tract. In case of accidental contact or inhalation, rinse immediately with plenty of water.
- To minimize the risk of aerosol contamination, operations should be conducted in separate designated areas whenever possible.
Related:
- PrimeIAmp™ HotStart Bst P DNA/RNA LHNB LyoMix (70ºC reaction)
- PrimeIAmp™ HotStart Bst P DNA/RNA Basic & SYBR Green LyoMix (70ºC reaction)
- PrimeIAmp™ HotStart Bst P DNA/RNA LHNB Microbead (70ºC reaction)
- PrimeIAmp™ HotStart Bst P DNA/RNA Basic Microbead (70ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA Basic & SYBR Green LyoMix (65ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead (65ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA Basic Microbead (65ºC reaction)
Viewpoint
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
SBS Genetech: Leading the Next Generation Isothermal Amplification Technology
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
