PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead (65ºC reaction)
$740.00
$925.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: IHLBM-100 (for 100T)
Description
PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead, meticulously developed by SBS Genetech, is optimized for LAMP amplification and supports both DNA and RNA target detection. Built upon the rapid and highly tolerant Bst DNA/RNA polymerase, it delivers stable, rapid, and highly sensitive amplification. The integration of HotStart technology significantly reduces the risk of false positives.
The HotStart Bst DNA/RNA polymerase contains a thermally responsive aptamer that inhibits enzyme activity by more than 90% at temperatures below 30 °C and fully restores activity within one minute at temperatures above 60 °C. This property allows for convenient room temperature setup and greatly reduces nonspecific amplification under low-temperature conditions.
In addition, the reagent incorporates LHNB (Leuco-HNB), a chromogenic dye that forms a deep violet complex with Mg²⁺ before the reaction. As the LAMP reaction proceeds, the accumulation of pyrophosphate (PPi) binds Mg²⁺, causing the color to shift from deep violet to pale green, visually indicating successful amplification.
(LHNB–Mg²⁺ complex) → (PPi–Mg²⁺) + (free LHNB)
Deep Violet Pale Green
Components
- PrimeIAmp™ HotStart Bst DNA/RNA LHNB Basic Microbead: 100Tx20μl
Storage:
- Store at –20 °C in a dry, sealed condition for up to 24 months. Alternatively, it can be stored at room temperature (25 °C) in a sealed container for up to 3 months.
- As the product is in a lyophilized form and highly hygroscopic, it is recommended to store it at –20 °C for up to 12 months after opening.
Precautions
- This product contains a high concentration of salts. Appropriate personal protective equipment (PPE) should be worn to avoid contact with skin, eyes, nose, and respiratory tract. In case of accidental contact or inhalation, rinse immediately with plenty of water.
- To minimize the risk of aerosol contamination, operations should be conducted in separate designated areas whenever possible.
Related:
- PrimeIAmp™ HotStart Bst P DNA/RNA LHNB LyoMix (70ºC reaction)
- PrimeIAmp™ HotStart Bst P DNA/RNA Basic & SYBR Green LyoMix (70ºC reaction)
- PrimeIAmp™ HotStart Bst P DNA/RNA LHNB Microbead (70ºC reaction)
- PrimeIAmp™ HotStart Bst P DNA/RNA Basic Microbead (70ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA Basic & SYBR Green LyoMix (65ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead (65ºC reaction)
- PrimeIAmp™ HotStart Bst DNA/RNA Basic Microbead (65ºC reaction)
Viewpoint
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
SBS Genetech: Leading the Next Generation Isothermal Amplification Technology
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
