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Cat. No.: IHPLM-100 (for 100T)

Description

PrimeIAmp™ HotStart Bst P DNA/RNA LHNB Microbead is a fully integrated lyophilized microsphere formulation, containing HotStart Bst P DNA/RNA polymerase, helicase, dNTPs, Mg²⁺, reaction buffer, LHNB colorimetric indicator (Leuco-HNB), and additional stabilizers.

Performance Features of Bst P:

1. HotStart Control: Bst P is equipped with a heat-activated aptamer that ensures >95% inhibition of enzyme activity below 30 °C and complete activation within 1 minute above 60 °C. This property supports room-temperature setup and significantly reduces nonspecific amplification under low-temperature conditions.

2. High-Temperature Amplification (70 °C): The elevated operating temperature minimizes primer dimer formation, improves amplification specificity, and enhances nucleic acid release from crude samples.

3. Helicase-Enabled eLAMP (Easy LAMP): The inclusion of helicase allows LAMP amplification without requiring F3/B3 outer primers and enables halving the amount of FIP/BIP primers. This helps further reduce nonspecific amplification and greatly improves amplification uniformity.

LHNB, the embedded chromogenic dye, initially forms a deep violet complex with Mg²⁺. As the LAMP reaction proceeds, pyrophosphate (PPi) generated during amplification sequesters Mg²⁺, leading to the release of LHNB and a visible color shift from deep violet to pale green.

(LHNB–Mg²⁺ complex) → (PPi–Mg²⁺) + (free LHNB)  

Deep Violet                  Pale Green

Components

• PrimeIAmp™ HotStart P Bst DNA/RNA LHNB Microbead: 100Tx20μl