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Cat. No.: DPPP-800 (for 800Tx25µ)

Description

The DP‑Probe (Displaceable Probe) is a strand‑displacement fluorescent probe designed specifically for fluorescence‑based LAMP amplification. When used together with HotStart Bst P Basic reagents, the Loop DP‑Probe delivers exceptional specificity and significantly reduces nonspecific amplification.

The Loop DP‑Probe Pair is generated by modifying one of the Loop primers (either LF or LB). It consists of two annealed, labeled oligonucleotides:

• a quencher probe (5’ IBFQ + Tail + Loop), and

• a reporter probe (Tail‑complementary region + 3’ fluorophore).

The annealed pair is non‑fluorescent. During the LAMP reaction, the Loop primer invades the “dumbbell” structure, extends during amplification, and displaces the reporter probe, which is released into solution and accumulates as a fluorescent signal. For detailed mechanistic information, please refer to PMID: 33626039.

Components

• 25xLAMP Loop DP-Probe Pair

Storage 

Store at −20 °C for up to 12 months. Aliquot upon receipt and avoid repeated freeze–thaw cycles.

Related:

• PrimeIAmp™ HotStart Bst P DNA/RNA LHNB LyoMix (70ºC reaction)​
• PrimeIAmp™ HotStart Bst P DNA/RNA Basic & SYBR Green LyoMix (70ºC reaction)
• PrimeIAmp™ HotStart Bst P DNA/RNA LHNB Microbead (70ºC reaction)
• PrimeIAmp™ HotStart Bst P DNA/RNA Basic Microbead (70ºC reaction)
• PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)
• PrimeIAmp™ HotStart Bst DNA/RNA Basic & SYBR Green LyoMix (65ºC reaction)
• PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead (65ºC reaction)
• PrimeIAmp™ HotStart Bst DNA/RNA Basic Microbead (65ºC reaction)
• 3M Spherical Lyophilization Excipient
• 1.67x Primer Bead Lyophilization Excipient
• LAMP Loop DP-Probe Pair

Only for research and not intended for treatment of humans or animals

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