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Cat. No.: T2HT2IC-20 (for 20 ea)

T-2 toxin (T-2) is a mycotoxin produced by various Fusarium species. It mainly contaminates cereals such as wheat, barley, maize, and their products, posing significant risks to human health and livestock farming. HT-2 toxin is the main metabolite of T-2 toxin. These toxins are potent inhibitors of protein synthesis in the body, primarily affecting the functions of blood, liver, kidney, pancreas, muscles, and lymphocytes. Typical clinical symptoms of poisoning include anorexia, vomiting, diarrhea, growth retardation, reproductive issues, and neurological disorders.

T-2/HT-2 Toxin Immunoaffinity Column selectively adsorbs T-2 and HT-2 toxins from the sample solution, thereby achieving highly targeted purification of the sample. After column purification, the sample solution can be directly used for LC-MS analysis after nitrogen evaporation or for HPLC analysis after derivatization.

Combining the immunoaffinity column with LC-MS and HPLC enables rapid determination, improving signal-to-noise ratio, and enhancing the accuracy of detection.

Principle

The basis of the assay is the antigen-antibody reaction, where antibodies are immobilized within the column. After sample pretreatment, the sample slowly passes through the immunoaffinity column, where toxins bind to the antibodies inside the column. Subsequently, the immunoaffinity column is washed to remove any unbound irrelevant substances. T-2/HT-2 toxins are eluted using 2% acetic acid-methanol solution and then injected into the analytical instrument for detection, either directly or after derivatization.

Precautions

• Before use, the immunoaffinity column should be equilibrated to room temperature (22-25℃).
• Store the immunoaffinity column at 2-8℃, do not freeze.
• Do not use immunoaffinity columns past their expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased as needed, but note that the volume of the extraction liquid should be adjusted accordingly.
• Column capacity: The total column capacity of the T-2/HT-2 toxin immunoaffinity column is 2000ng. If the concentration of the toxin in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample should be appropriately reduced for retesting.
• pH: The pH of the sample solution applied to the column should be between 6 and 8. If it deviates from this range, adjust the pH using hydrochloric acid or sodium hydroxide.
• Consistency between the solvent and the mobile phase during machine detection can eliminate the solvent effect.
• T-2/HT-2 toxins are cytotoxic, gloves should be worn when handling.
• It is advisable to soak used containers and T-2/HT-2 toxin solutions in a sodium hypochlorite solution (5% v/v) overnight.

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Only for research and not intended for treatment of humans or animals

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