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Cat. No.: VIC-20 (for 20 ea)

Deoxynivalenol (DON), also known as vomitoxin, belongs to the trichothecene mycotoxin family produced by certain species of Fusarium fungi. Vomitoxin is commonly found in grains such as wheat, barley, and maize, typically at levels of mg/kg. Due to their high cytotoxicity and immunosuppressive properties, vomitoxins pose a health threat to humans and animals.

Vomitoxin Immunoaffinity Column selectively adsorbs deoxynivalenol (DON), also known as vomitoxin, from sample solutions, thereby providing highly targeted purification of the samples. The sample solution after column purification, upon nitrogen blow and reconstitution, can be directly used for HPLC/LC-MS/MS analysis.

The combination of immunoaffinity columns with HPLC or LC-MS/MS facilitates rapid determination, improving signal-to-noise ratio and enhancing the accuracy of detection methods.

Principle

The basis of the assay relies on antigen-antibody reactions, with antibodies immobilized within the column. After sample extraction and filtration, the sample slowly passes through the immunoaffinity column for deoxynivalenol, where the toxin binds to the antibodies immobilized within the column. Subsequently, washing removes any unbound substances from the immunoaffinity column. Deoxynivalenol is eluted with methanol, dried under nitrogen, and then reconstituted before injection into the analytical instrument for detection.

Precautions

• Before use, the immunoaffinity column must be equilibrated to room temperature (22-25°C).
• Store the affinity column at 2-8°C; do not freeze.
• Do not use immunoaffinity columns that have exceeded their expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased according to requirements, but ensure that the volume of the extraction solution is adjusted accordingly.
• pH: The pH of the sample solution loaded onto the affinity column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Column capacity: 1500 ng. If the content of the toxin to be detected in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample solution should be appropriately reduced for retesting.
• Consistency between solvents and mobile phases during machine detection can eliminate the solvent effect.
• Vomiting toxin can be carcinogenic, so protective gear such as gloves and masks should be worn during operation.
• It is advisable to soak used containers and standard solution solutions overnight in a sodium hypochlorite solution (5% V/V).

Related:

• Zearalenone A Immunoaffinity Column
• Zearalenone Immunoaffinity Column
• Vitamin B12 (VB12) Immunoaffinity Column
• Vomitoxin Immunoaffinity Column
• Aflatoxin B1/B2/G1/G2 Immunoaffinity Column
• Aflatoxin M1 Immunoaffinity Column
• Aflatoxin M1&M2 Immunoaffinity Column
• Aflatoxin B1 Immunoaffinity Column
• Heparin Immunoaffinity Column
• Fumonisins B1/B2/B3 Immunoaffinity Column
• Protein G Immunoaffinity Column
• T-2 Toxin Immunoaffinity Column
• T-2/HT-2 Toxin Immunoaffinity Column
• Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column
• Fungal Toxin 3-in-1 (AFT Total, ZEN, DON) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFT, OTA) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column

Only for research and not intended for treatment of humans or animals

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