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Cat. No.: 2AZIC-20 (for 20 ea)

Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column can simultaneously adsorb aflatoxin B1 (AFB1) and zearalenone (ZEN) from the sample extract, thus achieving highly targeted purification of these two toxins. The purified sample liquid after column purification can be used for LC-MS (LC-MS/MS) analysis.

Principle

The basis of the assay is the antigen-antibody reaction, where antibodies for both toxins are simultaneously immobilized within the column. After the sample is extracted and filtered, it slowly passes through the immunoprecipitation column, where the toxins bind to their respective antibodies inside the column. Subsequently, the column is washed to remove any unbound irrelevant substances. The toxins are then eluted with methanol and injected into the analytical instrument for detection.

Precautions

• Before use, the immunoprecipitation column should be brought to room temperature (22-25°C).
• Store the column at 2-8°C, do not freeze.
• Please use the immunoprecipitation column before the expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased as needed, but be sure to adjust the volume of the extraction accordingly.
• pH: The pH of the sample solution applied to the column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Maintaining consistency between the solvent used for machine testing and the mobile phase can eliminate solvent effects.
• Column capacity:

• Both aflatoxin and zearalenone are toxic, so gloves should be worn when handling.
• It is recommended to soak used containers and toxin solutions in a sodium hypochlorite solution (5% v/v) overnight.

Related:

• Zearalenone A Immunoaffinity Column
• Zearalenone Immunoaffinity Column
• Vitamin B12 (VB12) Immunoaffinity Column
• Vomitoxin Immunoaffinity Column
• Aflatoxin B1/B2/G1/G2 Immunoaffinity Column
• Aflatoxin M1 Immunoaffinity Column
• Aflatoxin M1&M2 Immunoaffinity Column
• Aflatoxin B1 Immunoaffinity Column
• Heparin Immunoaffinity Column
• Fumonisins B1/B2/B3 Immunoaffinity Column
• Protein G Immunoaffinity Column
• T-2 Toxin Immunoaffinity Column
• T-2/HT-2 Toxin Immunoaffinity Column
• Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column
• Fungal Toxin 3-in-1 (AFT Total, ZEN, DON) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFT, OTA) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column

Only for research and not intended for treatment of humans or animals

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