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Cat. No.: AIC-20 (for 20 ea)

Aflatoxins are toxic metabolites produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. They are highly carcinogenic and are mainly found in grains, peanuts, nuts, cottonseed, feed, vegetable oils, as well as animal tissues and blood. Among them, aflatoxin B1 (AFT B1) has the highest toxicity, carcinogenicity, and contamination frequency.

Aflatoxin B1/B2/G1/G2 Immunoaffinity Column selectively adsorbs aflatoxins from the sample solution, thereby providing highly targeted purification of the sample. The sample solution after column purification can be directly used for HPLC analysis or detected with a fluorescence spectrophotometer after processing. Combining the affinity column with HPLC or a fluorescence spectrophotometer enables rapid determination, improving the signal-to-noise ratio and enhancing the accuracy of the detection method.

Principle

The assay is based on antigen-antibody reaction. Antibodies are immobilized within the column. Aflatoxins in the sample undergo extraction, filtration, and dilution. Then, the sample slowly passes through the aflatoxin immunoaffinity column. Within the column, the toxin binds to the antibodies. Subsequently, washing removes any unbound substances. Aflatoxins are then eluted with methanol and injected into the analytical instrument for detection.

Precautions

• Please use the immunoaffinity column within its expiration date.
• Before use, the immunoaffinity column should be equilibrated to room temperature (22-25℃).
• Store the affinity column at 2-8°C; do not freeze.
• Sample volume: The sample volume can be appropriately increased or decreased according to requirements, but ensure that the volume of the extraction solution is adjusted accordingly.
• Column capacity: 400 ng. If the content of the toxin to be detected in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample solution should be appropriately reduced for retesting.
• pH: The pH of the sample solution loaded onto the affinity column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Consistency between solvents and mobile phases during machine detection can eliminate the solvent effect.
• Aflatoxin B1 can be carcinogenic, so protective gear such as gloves and masks should be worn during operation.
• It is advisable to soak used containers and solutions containing aflatoxin B1 overnight in a sodium hypochlorite solution (5% V/V).

Related:

• Zearalenone A Immunoaffinity Column
• Zearalenone Immunoaffinity Column
• Vitamin B12 (VB12) Immunoaffinity Column
• Vomitoxin Immunoaffinity Column
• Aflatoxin B1/B2/G1/G2 Immunoaffinity Column
• Aflatoxin M1 Immunoaffinity Column
• Aflatoxin M1&M2 Immunoaffinity Column
• Aflatoxin B1 Immunoaffinity Column
• Heparin Immunoaffinity Column
• Fumonisins B1/B2/B3 Immunoaffinity Column
• Protein G Immunoaffinity Column
• T-2 Toxin Immunoaffinity Column
• T-2/HT-2 Toxin Immunoaffinity Column
• Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column
• Fungal Toxin 3-in-1 (AFT Total, ZEN, DON) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFT, OTA) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column

Only for research and not intended for treatment of humans or animals

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