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Cat. No.: AM1IC-20 (for 20 ea)

Aflatoxin M1 is a hydroxylated metabolite of aflatoxin B1 and is also a potent carcinogen. Milk and dairy products are susceptible to contamination by aflatoxin M1.

Aflatoxin M1 Immunoaffinity Column selectively adsorbs aflatoxin M1 from the sample solution, thereby providing highly targeted purification of the sample. The sample solution after column purification can be directly used for HPLC analysis.

Principle

The assay is based on antigen-antibody reaction. Antibodies are immobilized within the column. After centrifugation and defatting, the sample slowly passes through the aflatoxin M1 immunoaffinity column. Within the column, the toxin binds to the antibodies. Subsequently, washing removes any unbound substances. Aflatoxin M1 is then eluted with methanol and injected into the analytical instrument for detection.

Precautions

• Before use, the immunoaffinity column must be equilibrated to room temperature (22-25℃).
• Store the affinity column at 2-8°C; do not freeze.
• Do not use immunoaffinity columns that have exceeded their expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased according to requirements, but ensure that the volume of the extraction solution is adjusted accordingly.
• Column capacity: 150 ng. If the content of the toxin to be detected in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample solution should be appropriately reduced.
• pH: The pH of the sample solution loaded onto the affinity column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Consistency between solvents and mobile phases during machine detection can eliminate the solvent effect.
• Aflatoxin M1 can be carcinogenic, so gloves should be worn during operation.
• It is advisable to soak used containers and solutions containing aflatoxin M1 overnight in a sodium hypochlorite solution (5% V/V).
• When powdered milk samples are difficult to dissolve, they can be left to stand at 50°C for 30 minutes. If they still do not dissolve, the proportion of water used for dissolution should be increased appropriately.

Related:

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• Zearalenone Immunoaffinity Column
• Vitamin B12 (VB12) Immunoaffinity Column
• Vomitoxin Immunoaffinity Column
• Aflatoxin B1/B2/G1/G2 Immunoaffinity Column
• Aflatoxin M1 Immunoaffinity Column
• Aflatoxin M1&M2 Immunoaffinity Column
• Aflatoxin B1 Immunoaffinity Column
• Heparin Immunoaffinity Column
• Fumonisins B1/B2/B3 Immunoaffinity Column
• Protein G Immunoaffinity Column
• T-2 Toxin Immunoaffinity Column
• T-2/HT-2 Toxin Immunoaffinity Column
• Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column
• Fungal Toxin 3-in-1 (AFT Total, ZEN, DON) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFT, OTA) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column

Only for research and not intended for treatment of humans or animals

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