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Cat. No.: 3BZTIC-20 (for 20 ea)

Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column can simultaneously adsorb aflatoxin B1 (AFB1), zearalenone (ZEN), and T-2 toxin (T-2) from the sample extract, thereby achieving highly targeted purification of these three toxins. The purified sample liquid after passing through the column can be used for LC-MS (LC-MS/MS) analysis.

Principle

The basis of the assay is the antigen-antibody reaction, where antibodies against the three toxins are simultaneously bound within the column. After the sample is extracted and filtered, it slowly passes through the immunoaffinity column, where the toxins bind to their respective antibodies inside the column. Subsequently, the immunoaffinity column is washed to remove any unbound irrelevant substances. The toxins are then eluted with methanol and injected into the analytical instrument for detection.

Precautions

• Before use, the immunoaffinity column should be brought to room temperature (22-25°C).
• Store the column at 2-8°C, do not freeze.
• Please use the immunoaffinity column within its expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased as needed, but the volume of the extraction solution should be adjusted accordingly.
• pH: The pH of the sample solution for the immunoaffinity column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Consistency between the solvent used during machine testing and the mobile phase can eliminate solvent effects.
• Column capacity:

• Aflatoxin B1, Zearalenone, and T-2 toxin are all toxic, so gloves should be worn when handling.
• It is preferable to soak used containers and toxin solutions overnight in a 5% sodium hypochlorite solution.

Related:

• Zearalenone A Immunoaffinity Column
• Zearalenone Immunoaffinity Column
• Vitamin B12 (VB12) Immunoaffinity Column
• Vomitoxin Immunoaffinity Column
• Aflatoxin B1/B2/G1/G2 Immunoaffinity Column
• Aflatoxin M1 Immunoaffinity Column
• Aflatoxin M1&M2 Immunoaffinity Column
• Aflatoxin B1 Immunoaffinity Column
• Heparin Immunoaffinity Column
• Fumonisins B1/B2/B3 Immunoaffinity Column
• Protein G Immunoaffinity Column
• T-2 Toxin Immunoaffinity Column
• T-2/HT-2 Toxin Immunoaffinity Column
• Fungal Toxin 3-in-1 (B1, ZEN, T-2) Immunoaffinity Column
• Fungal Toxin 3-in-1 (AFT Total, ZEN, DON) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFT, OTA) Immunoaffinity Column
• Fungal Toxin 2-in-1 (AFB1, ZEN) Immunoaffinity Column

Only for research and not intended for treatment of humans or animals

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