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Cat. No.: T2IC-20 (for 20 ea)

T-2 toxin (T-2) is a mycotoxin produced by various Fusarium species. It mainly contaminates cereal crops such as wheat, barley, maize, and their products, posing significant risks to human health and animal husbandry. T-2 toxin primarily affects the function of blood, liver, kidney, pancreatic muscles, and lymphocytes. General clinical symptoms of T-2 toxin poisoning include anorexia, vomiting, diarrhea, growth retardation, reproductive and neurological dysfunction, among others.

The immunoaffinity column selectively adsorbs T-2 toxin from the sample solution, thereby providing highly targeted purification of the sample. The sample solution purified through the column can be directly used for LC-MS/MS analysis after nitrogen evaporation and reconstitution or for HPLC analysis after derivatization.

Combining the immunoaffinity column with LC-MS/MS and HPLC allows for rapid determination, improving the signal-to-noise ratio and enhancing the accuracy of the detection method.

Principle

The basis of the assay is the antigen-antibody reaction, where the antibody is immobilized within the column. After sample pre-treatment, the sample slowly passes through the T-2 toxin immunoaffinity column, where the toxin binds to the antibody inside the column. Subsequently, the immunoaffinity column is washed to remove any unbound irrelevant substances. T-2 toxin is eluted using 2% acetic acid-methanol solution, which is then injected into the analytical instrument for detection either directly or after derivatization.

Precautions

• Before use, the immunoaffinity column must be brought to room temperature (22-25℃).
• Store the column at 2-8℃, do not freeze.
• Please use the immunoaffinity column before the expiration date.
• Sample volume: Adjust the sample volume accordingly based on the requirements, ensuring corresponding adjustments in the extraction volume.
• Column capacity: 2000ng. If the content of toxins to be tested in the sample divided by the dilution factor exceeds the column capacity, it is necessary to appropriately reduce the volume of the sample for retesting.
• pH: The pH of the sample solution applied to the column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Consistency between the solvent and mobile phase during machine detection can eliminate solvent effects.
• T-2 toxin is cytotoxic, so protective equipment such as gloves and masks should be worn during handling.
• It is recommended to soak used containers and T-2 toxin solutions overnight in a 5% sodium hypochlorite solution.

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Only for research and not intended for treatment of humans or animals

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