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- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
from China, for the World
for Superior Biology Services since 2000
- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
- …
- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
PCR-Related
At SBS Genetech, we are at the forefront of offering comprehensive solutions for PCR-related products, from basic to advanced
Polymerase Chain Reaction Basics
Start with four main components of a Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. There are four main components required for a reaction: DNA template, DNA primers, free nucleotides called dNTPs, and DNA polymerase (e.g. U-Taq DNA Polymerase). At SBS Genetech, we are at the forefront of offering comprehensive solutions for PCR-Related products based on our various platforms.
Gene synthesis is an efficient and cost-effective alternative to molecular cloning for getting your DNA template. We can synthesize codon-optimized cDNA, gene variants, artificially designed DNA, or any other sequence for your research.
Our oligos are synthesized with a typical coupling efficiency of 99% and purified by QPC free of charge. PAGE purification is also available with an additional charge. The high coupling efficiency of our synthesis technology allows efficient synthesis of oligos up to 120 bases.
We offer comprehensive DNA polymerases of high quality, from basic U-Taq DNA Polymerase and Pfu DNA Polymerase to PrimeTaq™ HotStart Direct PCR DNA Polymerase, which features hot start property and high tolerance to many PCR inhibitors, an ideal enzyme qualified for almost all scenarios.
Advanced Solutions
We continually push the boundaries of PCR technology through enzyme innovation
UniProof HiFi DNA Polymerase
A high-fidelity, uracil-tolerant DNA polymerase engineered for robust long-fragment amplification, efficient uracil-containing template compatibility, and streamlined RT-PCR and USER cloning workflows.
Derived from a high-fidelity DNA polymerase backbone, UniProof incorporates an optimized extension domain for robust long-fragment amplification and high yield. Through intensive screening of the exonuclease domain, it achieves exceptional fidelity—comparable to Q5—with 280× the fidelity of Taq polymerase. Engineering at the substrate-binding center confers tolerance to dUTP and enables efficient amplification of uracil-containing templates.
UniProof readily amplifies up to 8 kb of genomic DNA or 20 kb of λDNA, boasting an extension rate exceeding 2 kb/min. The enzyme exhibits strong 5’→3’ polymerase activity and robust 3’→5’ exonuclease (proofreading) activity, producing blunt-end products.
Unlike many B-family high-fidelity polymerases that stall at uracil residues in the template strand, UniProof lacks a uracil-binding domain, enabling it to read and amplify uracil-containing DNA. Additionally, UniProof is widely used in USER cloning workflows.
CM HS-G3Taq DNA Polymerase (Glycerol Free)
Features chemical inactivation below 50°C to enhance PCR specificity and sensitivity, supported by an optimized proprietary buffer for robust, high-yield amplification.
G3Taq DNA Polymerase is a third-generation Taq DNA polymerase featuring 5’→3’ exonuclease activity but lacking 3’→5’ exonuclease (proofreading) activity. It exhibits exceptional tolerance to impurities such as ethanol, guanidine salts, and heparin, enabling it to deliver reliable results even with low-purity DNA templates.
CM HS-G3Taq DNA Polymerase is a chemically modified HotStart version of the enzyme. It remains completely inactive below 50°C and regains full activity only after heating at 95°C for 5 minutes. This feature effectively suppresses nonspecific amplification and significantly enhances PCR specificity and sensitivity.
The enzyme is supplied with a proprietary reaction buffer, optimized to support robust amplification across a broad range of conditions, yielding higher sensitivity and stronger signal intensity.
5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
A dual aptamer-blocked HotStart master mix enabling high-sensitivity, Mn²⁺-free DNA/RNA amplification directly from crude samples, powered by G3Taq DNA Polymerase and Extreme ThermoStable Reverse Transcriptase (ET RTase).
5× DualHS G3mTTx qPCR Mix is a specialized 5× master mix for TaqMan-based Real-Time PCR, featuring dual aptamer-based HotStart enzymes—G3Taq DNA Polymerase and Extreme ThermoStable Reverse Transcriptase (ET RTase)—along with dNTPs, Mg²⁺ activator, Mg²⁺ ions, and stabilizers. The mix enables bias-free amplification of both DNA and RNA under Mg²⁺-dependent conditions (no Mn²⁺ required) and supports ultrasensitive detection of nucleic acids.
Due to the extreme heat resistance of ET RTase (retaining activity after 95°C treatment), the system allows direct detection of crude samples—thermal lysis efficiently releases nucleic acids from both DNA and RNA viruses, eliminating the need for nucleic acid purification prior to amplification.
Auxiliary Tools
We provide powerful auxiliary tools to make your PCR experiments smoother
Heat-Labile Uracil DNA Glycosylase
Heat-Labile Uracil-DNA Glycosylase (HL-UDG) is a recombinant protein expressed by E. coli which is originally from psychrotrophic marine bacteria. HL-UDG can achieve optimal anti-contamination ability when adding to the PCR system and can be irreversibly inactivated by heat denaturation at 95°C for 5 min later, without affecting subsequent PCR reactions. Based on its unique characteristic, we have developed PrimeTaq™ Probe qPCR MasterMix (with HL-UDG), which has excellent performance against nucleic acid aerosol contamination.Taq Monoclonal Antibody
Taq Monoclonal Antibody is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. Taq Monoclonal Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing primer dimer formation and non-specific amplification resulted from non-specific priming at ambient temperature before the onset of thermal cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step.5 × GC Solution
GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer-dimer and nonspecific amplification and thus improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary. The minimum shelf life is 2 years at -20°C.Ready for this new experience?
PCR-Related Products
Buy nowT7 ARCA in vitro mRNA Synthesis Kit$800.00 - $2,000.00$2,500.00Buy nowGoodView™ Ultra Nucleic Acid Stain$840.00 - $7,000.00$10,000.00Buy nowGoodView™ Red Nucleic Acid Stain$420.00 - $3,500.00$5,000.00Buy nowGoodView™ Green Nucleic Acid Stain$420.00 - $3,500.00$5,000.00Buy nowHotstart Probe One-Step qRT-PCR Kit$160.00 - $600.00$750.00Buy nowHotstart Probe qPCR Mix (2X)$160.00 - $680.00$850.00Buy nowSurrogate GFP Reporter Construction Kit$680.00$850.00Buy nowdITP (100mM)$95.00 - $295.00Buy now3'-ONH₂-dATP (100mM Solution) | 1220515-87-6$700.00$1,000.00Buy now3'-ONH₂-dGTP (100mM Solution) | 1220515-98-9$700.00$1,000.00Buy now3'-ONH₂-dCTP (100mM Solution) | 1220515-75-2$700.00$1,000.00Buy now3'-ONH₂-dTTP (100mM Solution) | 1159990-37-0$700.00$1,000.00More Information
August 31, 2024Read more...Designing PCR (Polymerase Chain Reaction) primers is a pivotal aspect of molecular biology...Read more...In the vast landscape of molecular biology, where understanding the intricacies of DNA and RNA is...Read more...In the realm of molecular biology, the ability to accurately detect and quantify specific DNA...- ContactFor more information, please fill out the provided form or contact us directly by E-mail
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from China, for the World
for Superior Biology Services since 2000