Derived from a high-fidelity DNA polymerase backbone, UniProof incorporates an optimized extension domain for robust long-fragment amplification and high yield. Through intensive screening of the exonuclease domain, it achieves exceptional fidelity—comparable to Q5—with 280× the fidelity of Taq polymerase. Engineering at the substrate-binding center confers tolerance to dUTP and enables efficient amplification of uracil-containing templates.

UniProof readily amplifies up to 8 kb of genomic DNA or 20 kb of λDNA, boasting an extension rate exceeding 2 kb/min. The enzyme exhibits strong 5’→3’ polymerase activity and robust 3’→5’ exonuclease (proofreading) activity, producing blunt-end products.

Unlike many B-family high-fidelity polymerases that stall at uracil residues in the template strand, UniProof lacks a uracil-binding domain, enabling it to read and amplify uracil-containing DNA. Additionally, UniProof is widely used in USER cloning workflows.

5×​ DualHS G3mTTx qPCR Mix is a specialized 5× master mix for TaqMan-based Real-Time PCR, featuring dual aptamer-based HotStart enzymes—G3Taq DNA Polymerase and Extreme ThermoStable Reverse Transcriptase (ET RTase)—along with dNTPs, Mg²⁺ activator, Mg²⁺ ions, and stabilizers. The mix enables bias-free amplification of both DNA and RNA under Mg²⁺-dependent conditions (no Mn²⁺ required) and supports ultrasensitive detection of nucleic acids.

Due to the extreme heat resistance of ET RTase (retaining activity after 95°C treatment), the system allows direct detection of crude samples—thermal lysis efficiently releases nucleic acids from both DNA and RNA viruses, eliminating the need for nucleic acid purification prior to amplification.