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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
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from China, for the World

for Superior Biology Services since 2000

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  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
  •  

    PCR-Related

    At SBS Genetech, we are at the forefront of offering comprehensive solutions for PCR-related products, from basic to advanced

  • Polymerase Chain Reaction Basics

    Start with four main components of a Polymerase Chain Reaction

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    Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. There are four main components required for a reaction: DNA template, DNA primers, free nucleotides called dNTPs, and DNA polymerase (e.g. U-Taq DNA Polymerase). At SBS Genetech, we are at the forefront of offering comprehensive solutions for PCR-Related products based on our various platforms.

  • DNA template

    Gene synthesis is an efficient and cost-effective alternative to molecular cloning for getting your DNA template. We can synthesize codon-optimized cDNA, gene variants, artificially designed DNA, or any other sequence for your research.

    DNA primers

    Our oligos are synthesized with a typical coupling efficiency of 99% and purified by QPC free of charge. PAGE purification is also available with an additional charge. The high coupling efficiency of our synthesis technology allows efficient synthesis of oligos up to 120 bases.

    dNTPs

    Our dNTPs are available as ready-to-use mix or sets. Both are free of RNase and DNase and are suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis, and nick translation. Purity is more than 99%.

    DNA polymerase​

    We offer comprehensive DNA polymerases of high quality, from basic U-Taq DNA Polymerase and Pfu DNA Polymerase to PrimeTaq™ HotStart Direct PCR DNA Polymerase, which features hot start property and high tolerance to many PCR inhibitors, an ideal enzyme qualified for almost all scenarios.

  • Advanced Solutions

    We continually push the boundaries of PCR technology through enzyme innovation

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    UniProof HiFi DNA Polymerase

    A high-fidelity, uracil-tolerant DNA polymerase engineered for robust long-fragment amplification, efficient uracil-containing template compatibility, and streamlined RT-PCR and USER cloning workflows.

    Derived from a high-fidelity DNA polymerase backbone, UniProof incorporates an optimized extension domain for robust long-fragment amplification and high yield. Through intensive screening of the exonuclease domain, it achieves exceptional fidelity—comparable to Q5—with 280× the fidelity of Taq polymerase. Engineering at the substrate-binding center confers tolerance to dUTP and enables efficient amplification of uracil-containing templates.

    UniProof readily amplifies up to 8 kb of genomic DNA or 20 kb of λDNA, boasting an extension rate exceeding 2 kb/min. The enzyme exhibits strong 5’→3’ polymerase activity and robust 3’→5’ exonuclease (proofreading) activity, producing blunt-end products.

    Unlike many B-family high-fidelity polymerases that stall at uracil residues in the template strand, UniProof lacks a uracil-binding domain, enabling it to read and amplify uracil-containing DNA. Additionally, UniProof is widely used in USER cloning workflows.

    Learn More
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    CM HS-G3Taq DNA Polymerase (Glycerol Free)

    Features chemical inactivation below 50°C to enhance PCR specificity and sensitivity, supported by an optimized proprietary buffer for robust, high-yield amplification.

    G3Taq DNA Polymerase is a third-generation Taq DNA polymerase featuring 5’→3’ exonuclease activity but lacking 3’→5’ exonuclease (proofreading) activity. It exhibits exceptional tolerance to impurities such as ethanol, guanidine salts, and heparin, enabling it to deliver reliable results even with low-purity DNA templates.

    CM HS-G3Taq DNA Polymerase is a chemically modified HotStart version of the enzyme. It remains completely inactive below 50°C and regains full activity only after heating at 95°C for 5 minutes. This feature effectively suppresses nonspecific amplification and significantly enhances PCR specificity and sensitivity.

    The enzyme is supplied with a proprietary reaction buffer, optimized to support robust amplification across a broad range of conditions, yielding higher sensitivity and stronger signal intensity.

    Learn More
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    5×​ DualHS G3mTTx qPCR Mix (Lyo-Ready)

    A dual aptamer-blocked HotStart master mix enabling high-sensitivity, Mn²⁺-free DNA/RNA amplification directly from crude samples, powered by G3Taq DNA Polymerase and Extreme ThermoStable Reverse Transcriptase (ET RTase).

    5×​ DualHS G3mTTx qPCR Mix is a specialized 5× master mix for TaqMan-based Real-Time PCR, featuring dual aptamer-based HotStart enzymes—G3Taq DNA Polymerase and Extreme ThermoStable Reverse Transcriptase (ET RTase)—along with dNTPs, Mg²⁺ activator, Mg²⁺ ions, and stabilizers. The mix enables bias-free amplification of both DNA and RNA under Mg²⁺-dependent conditions (no Mn²⁺ required) and supports ultrasensitive detection of nucleic acids.

    Due to the extreme heat resistance of ET RTase (retaining activity after 95°C treatment), the system allows direct detection of crude samples—thermal lysis efficiently releases nucleic acids from both DNA and RNA viruses, eliminating the need for nucleic acid purification prior to amplification.

    Learn More
  • Auxiliary Tools

    We provide powerful auxiliary tools to make your PCR experiments smoother

  • Heat-Labile Uracil DNA Glycosylase

    Heat-Labile Uracil-DNA Glycosylase (HL-UDG) is a recombinant protein expressed by E. coli which is originally from psychrotrophic marine bacteria. HL-UDG can achieve optimal anti-contamination ability when adding to the PCR system and can be irreversibly inactivated by heat denaturation at 95°C for 5 min later, without affecting subsequent PCR reactions. Based on its unique characteristic, we have developed PrimeTaq™ Probe qPCR MasterMix (with HL-UDG), which has excellent performance against nucleic acid aerosol contamination.

    Learn More

    Taq Monoclonal Antibody

    Taq Monoclonal Antibody is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. Taq Monoclonal Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing primer dimer formation and non-specific amplification resulted from non-specific priming at ambient temperature before the onset of thermal cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step.

    Learn More

    5 × GC Solution

    GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer-dimer and nonspecific amplification and thus improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary. The minimum shelf life is 2 years at -20°C.

    Learn More
  • Ready for this new experience?

  • PCR-Related Products

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    Buy now
    5×​ DualHS G3mTTx qPCR Mix (Lyo-Ready)
    $300.00 - $1,200.00
    $1,500.00
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    Buy now
    AP HS-G3Taq DNA Polymerase (Glycerol Free)
    $80.00 - $240.00
    $300.00
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    5×​ CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
    $300.00 - $1,200.00
    $1,500.00
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    5× CM HS-G3Taq Probe Mix (Lyo-Ready)
    $160.00 - $600.00
    $750.00
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    5× CM HS-G3Taq SYBR Green qPCR Mix
    $120.00 - $480.00
    $600.00
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    Buy now
    CM HS-G3Taq DNA Polymerase (Glycerol Free)
    $80.00 - $240.00
    $300.00
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    Buy now
    5× UniProof HiFi PCR Mix (with Blue Dye)
    $180.00 - $1,400.00
    $1,750.00
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    UniProof HiFi DNA Polymerase
    $200.00 - $800.00
    $1,000.00
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    Buy now
    T7 ARCA in vitro mRNA Synthesis Kit
    $800.00 - $2,000.00
    $2,500.00
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    Buy now
    GoodView™ Ultra Nucleic Acid Stain
    $840.00 - $7,000.00
    $10,000.00
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    GoodView™ Red Nucleic Acid Stain
    $420.00 - $3,500.00
    $5,000.00
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    GoodView™ Green Nucleic Acid Stain
    $420.00 - $3,500.00
    $5,000.00
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    Hotstart Probe One-Step qRT-PCR Kit
    $160.00 - $600.00
    $750.00
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    Hotstart Probe qPCR Mix (2X)
    $160.00 - $680.00
    $850.00
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    Surrogate GFP Reporter Construction Kit
    $680.00
    $850.00
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    dITP (100mM)
    $95.00 - $295.00
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    3'-ONH₂-dATP (100mM Solution) | 1220515-87-6
    $700.00
    $1,000.00
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    Buy now
    3'-ONH₂-dGTP (100mM Solution) | 1220515-98-9
    $700.00
    $1,000.00
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    Buy now
    3'-ONH₂-dCTP (100mM Solution) | 1220515-75-2
    $700.00
    $1,000.00
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    Buy now
    3'-ONH₂-dTTP (100mM Solution) | 1159990-37-0
    $700.00
    $1,000.00
  • More Information

    Mastering PCR Primer Design: The 12 Golden Rules for Success
    August 31, 2024
    Designing PCR (Polymerase Chain Reaction) primers is a pivotal aspect of molecular biology...
    Read more...
    Exploring the Key Players in Nucleic Acid Synthesis: NTP vs dNTP vs ddNTP
    August 30, 2024
    In the vast landscape of molecular biology, where understanding the intricacies of DNA and RNA is...
    Read more...
    Understanding TaqMan Probes and Molecular Beacons in Molecular Biology
    July 27, 2024
    In the realm of molecular biology, the ability to accurately detect and quantify specific DNA...
    Read more...
  • Cold Spring Harbor Laboratory - SBS Genetech

    SBS Genetech is a long-term sponsor of

    Cold Spring Harbor Laboratory

    Learn More
  • Contact

    For more information, please fill out the provided form or contact us directly by E-mail

    tech@sbsbio.com

SBS Genetech © Copyright 2000-2025

from China, for the World

for Superior Biology Services since 2000

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