Polymerase Chain Reaction Basics
Start with four main components of a PCR DNA amplification reaction
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. There are four main components required for a reaction: DNA template, DNA primers, free nucleotides called dNTPs, and DNA polymerase (e.g. U-Taq DNA Polymerase). At SBS Genetech, we are at the forefront of offering comprehensive solutions for PCR-Related products based on our various platforms.
Advanced Solutions
With SBS Genetech's PCR-related products, you can almost do anything as long as you have an idea
Direct PCR using DNA as template
By PrimeTaq™ HotStart Direct PCR DNA Polymerase
PrimeTaq™ HotStart Direct PCR DNA Polymerase has hot start property and high tolerance to many PCR inhibitors. High tolerance delivers convenience for DNA amplification by allowing PCR directly from samples without prior DNA purification. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 95°C for 5 min. Therefore, the system can effectively inhibit non-specific PCR amplification, greatly improving the specificity and sensitivity. The polymerase has 5 '- 3' exonuclease activity, without 3 '- 5' exonuclease activity.Direct PCR using RNA as template
By PrimeTaq™ HS DP DNA/RNA Polymerase
PrimeTaq™ HS DP DNA/RNA Polymerase has hot start property and high tolerance to many PCR inhibitors. High tolerance delivers convenience for both DNA and RNA amplification by allowing PCR directly from samples without prior purification. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 92°C for 5 min. Therefore, the system can effectively inhibit non-specific PCR amplification, greatly improving the specificity and sensitivity.PCDR using DNA as template
By SD TM DNA Polymerase
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. SD TM DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity, which is particularly effective for TaqMan PCDR of DNA templates.PCDR using RNA as template
By SD TM DNA/RNA Polymerase
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. SD TM DNA/RNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement and reverse transcriptase activity. SD TM DNA/RNA polymerase is particularly effective for TaqMan PCDR of both DNA and RNA templates. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 90°C for 5 min.Genotyping of purified DNA samples
By PrimeSNP™ Genotyping Kit
PrimeSNP™ Genotyping Kit uses Competitive Allele Specific PCR method for genotyping of purified DNA samples. This method does not need to synthesize specific probes for each SNP and indel. Instead, it only requires two unique pairs of probes to achieve accurate genotyping of genomic DNA samples. By analyzing the intensity and ratio of terminal fluorescence signal, genotype is automatically discriminated, and the effect of clustering is intuitive. This kit has the advantages of less detection time, lower reagent cost and higher detection accuracy.Improving the specificity of the polymerase
By PrimeTaq™ Taq Monoclonal Antibody
PrimeTaq™ Taq Monoclonal Antibody is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. PrimeTaq™ Taq Monoclonal Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing primer dimer formation and non-specific amplification resulted from non-specific priming at ambient temperature before the onset of thermal cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step.
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