Start with four main components of a Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. There are four main components required for a reaction: DNA template, DNA primers, free nucleotides called dNTPs, and DNA polymerase (e.g. U-Taq DNA Polymerase). At SBS Genetech, we are at the forefront of offering comprehensive solutions for PCR-Related products based on our various platforms.
Gene synthesis is an efficient and cost-effective alternative to molecular cloning for getting your DNA template. We can synthesize codon-optimized cDNA, gene variants, artificially designed DNA, or any other sequence for your research.
Our oligos are synthesized with a typical coupling efficiency of 99% and purified by QPC free of charge. PAGE purification is also available with an additional charge. The high coupling efficiency of our synthesis technology allows efficient synthesis of oligos up to 120 bases.
Our dNTPs are available as ready-to-use mix or sets. Both are free of RNase and DNase and are suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis, and nick translation. Purity is more than 99%.
Define the enzyme for your research by following basic properties
Defining the ability to discriminate correct vs. incorrect nucleotide incorporation. The higher the fidelity means the better ability to "proofread".
Measuring the speed nucleotides are incorporated with suitable buffer and temperature. The higher extension rate means the faster amplification
PrimeTaq™ HiFi PCR Polymerase, which is derived from high fidelity DNA polymerase and added with enhanced extension structure, has super high fidelity (~ 280 times of Taq DNA Polymerase), long fragment amplification ability, and higher yield. The polymerase can easily amplify 8 kb genomic DNA, 20 kb λDNA, and 8 kb cDNA. The polymerase has an extension rate of more than 6 kb/min.
The Development of PCR
From basic chemical reaction to advanced statistical analysis
The 1st Generation
By comparing the intensity of the amplified PCR product on a gel to standards of a known concentration (usually using DNA Markers) at the end of the PCR cycles, the results of qualitative detection are obtained. Traditional PCR suffers from poor precision and low sensitivity.
The 2nd Generation
By collecting data during the exponential growth phase of PCR (e.g. signal from the fluorescent reporter), the quantity of the PCR product can be measured as it is directly proportional to the amount of starting template nucleic acid. Real-time PCR is very precise and specific.
The 3rd Generation
By measuring the fraction of negative replicates, the absolute copies (e.g. viral load) can be determined by Poisson statistical algorithm. Digital PCR provides a novel solution for absolute quantification and there are no references or standards required.
With PrimeTaq™ series, everything becomes easy and "direct"
Direct PCR of DNA from Crude Sample
By PrimeTaq™ HotStart Direct PCR DNA Polymerase
PrimeTaq™ HotStart Direct PCR DNA Polymerase has hot start property and high tolerance to many PCR inhibitors. High tolerance delivers convenience for DNA amplification by allowing PCR directly from samples without prior DNA purification. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 95°C for 5 min. Therefore, the system can effectively inhibit non-specific PCR amplification, greatly improving the specificity and sensitivity. The polymerase has 5 '- 3' exonuclease activity, without 3 '- 5' exonuclease activity.
PrimeTaq™ HS DP DNA/RNA Polymerase has hot start property and high tolerance to many PCR inhibitors. High tolerance delivers convenience for both DNA and RNA amplification by allowing PCR directly from samples without prior purification. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 92°C for 5 min. Therefore, the system can effectively inhibit non-specific PCR amplification, greatly improving the specificity and sensitivity.
PrimeTaq™ Probe qPCR MasterMix, which premixes reagents including chemically modified PrimeTaq™ HotStart Direct PCR DNA Polymerase, reaction buffer, and dNTPs. This MasterMix does not contain ROX dyes, which is compatible with a wide range of fluorescent probes and a variety of quantitative PCR instruments. In addition, this MasterMix has great performance in multiplex quantitative PCR (multiplex qPCR) assays, enabling a minimum of 4-plex quantitative PCR assays.
PrimeTaq™ Probe One-Step RT-qPCR MasterMix contains PrimeTaq™ HS DP DNA/RNA Polymerase, which has hot start property and high tolerance to many PCR inhibitors. High tolerance delivers convenience for both DNA and RNA amplification by allowing PCR directly from samples without prior purification. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 92°C for 5 min. Therefore, the system can effectively inhibit non-specific PCR amplification, greatly improving the specificity and sensitivity.
We provide powerful auxiliary tools to make your PCR experiments smoother
Heat-Labile Uracil DNA Glycosylase
Heat-Labile Uracil-DNA Glycosylase (HL-UDG) is a recombinant protein expressed by E. coli which is originally from psychrotrophic marine bacteria. HL-UDG can achieve optimal anti-contamination ability when adding to the PCR system and can be irreversibly inactivated by heat denaturation at 95°C for 5 min later, without affecting subsequent PCR reactions. Based on its unique characteristic, we have developed PrimeTaq™ Probe qPCR MasterMix (with HL-UDG), which has excellent performance against nucleic acid aerosol contamination.
Taq Monoclonal Antibody is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. Taq Monoclonal Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing primer dimer formation and non-specific amplification resulted from non-specific priming at ambient temperature before the onset of thermal cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step.
GC-Solution is an auxiliary reagent for gene amplification. It is mainly used for PCR amplification of high GC content templates (> 70%) and long fragments (> 4 kb). It can reduce primer-dimer and nonspecific amplification and thus improve amplification efficiency. GC-solution will affect the stability of primer-template pairing, so please don’t add it if unnecessary. The minimum shelf life is 2 years at -20°C.