PrimeTaq™ HotStart Direct PCR DNA Polymerase has hot start property and high tolerance to many PCR inhibitors. High tolerance delivers convenience for DNA amplification by allowing PCR directly from samples without prior DNA purification. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 95°C for 5 min. Therefore, the system can effectively inhibit non-specific PCR amplification, greatly improving the specificity and sensitivity. The polymerase has 5 '- 3' exonuclease activity, without 3 '- 5' exonuclease activity.