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Cat. No.: UPHF-200 (for 200U)

Cat. No.: UPHF-1k (for 1000U)

Description

UniProof HiFi DNA Polymerase is a versatile, all-in-one enzyme developed through our proprietary molecular enzyme evolution platform (in silico Design & in vitro Evolution Workflow). It combines the strengths of Q5 and Q5U polymerases, delivering enhanced performance across diverse applications.

Derived from a high-fidelity DNA polymerase backbone, UniProof incorporates an optimized extension domain for robust long-fragment amplification and high yield. Through intensive screening of the exonuclease domain, it achieves exceptional fidelity—comparable to Q5—with 280× the fidelity of Taq polymerase. Engineering at the substrate-binding center confers tolerance to dUTP and enables efficient amplification of uracil-containing templates.

UniProof readily amplifies up to 8 kb of genomic DNA or 20 kb of λDNA, boasting an extension rate exceeding 2 kb/min. The enzyme exhibits strong 5’→3’ polymerase activity and robust 3’→5’ exonuclease (proofreading) activity, producing blunt-end products.

Unlike many B-family high-fidelity polymerases that stall at uracil residues in the template strand, UniProof lacks a uracil-binding domain, enabling it to read and amplify uracil-containing DNA. This unique “U-tolerant” feature supports two key applications:

1. Ultra-high fidelity amplification of bisulfite-converted, deaminated, or damaged DNA templates

2. Contamination control in PCR systems using dUTP and heat-labile UDG

Additionally, UniProof is widely used in USER cloning workflows.

Features

• Ultra-high fidelity amplification: Fidelity equivalent to Q5 DNA Polymerase (280× the fidelity of Taq polymerase).
• dUTP compatibility: Capable of reading and amplifying uracil-containing templates, with a distinctive “U-tolerant” characteristic.
• Contamination control: Enables complete substitution of dTTP with dUTP for high-fidelity, contamination-resistant PCR amplification.
• Long-fragment amplification: Efficiently amplifies >20 kb from simple templates (e.g., plasmids, λDNA) and >8 kb from complex genomic DNA.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble material within 30 minutes at 72°C.

Storage Conditions

• Storage buffer: 10 mM Tris-HCl (pH 7.6), 100 mM NaCl, 0.05% Tween-20, 1 mM DTT, 0.5 mM EDTA, 50% glycerol.
• Storage temperature: Stable at –20°C for up to 3 years.

Related: 5× UniProof HiFi PCR Mix (with Blue Dye)

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory